从对CREB信号传导网络的调控作用探讨清热活血解毒法治疗寻常型银屑病的作用机制

Psoriasis is a chronic relapsing inflammatory skin disease with high incidence , the pathogenesis of psoriasis is still an enigma which immunity play a crual role in it .The typical clinical appearance is well-demarcated scaly erythematous macules covered with white silvery scales.It always makes seriously affection on the physical and mental health of patients.Lots of clinical trials show that the reatment of TCM is effective, but the mechanism of the need to be stutied...In the previous studies, we found that Qingre Jiedu Huoxue Decoction drug serum can inhibit the ptoliferation of a variety of psoriasis associated cellsin vitro, and affect the expression level of cAMP, CaM, NF-KB in keratinocytes. what is more,the above factors participate the signal transduction pathways of psoriasis, such as cAMP signaling pathway, Ca2+-CaMK, NF-KB signaling pathways. Therefore, we believe that the mechanism of TCM in treating psorisis is complex, it can make function through influence the sigaling network consisting by a number of signal transduction pathway...Even more, the cAMP element binding protein ( CREB ) is a transcription factor and a interwoven node of a plurality of psoriasis related signal transduction pathways. it can activate the release of downstream signaling factor falls. So we propose a new hypothes: CREB and its signal transduction networks may be a key target of treatment of TCM of psoriasis..

银屑病是一种多基因遗传背景下,受环境因素影响的免疫介导下发生的红斑鳞屑性皮肤疾病，发病率高，严重影响患者身心健康，临床研究显示中医药治疗银屑病是具有一定优势，然而其治疗的作用机制却有待于深入研究。前期研究中，我们发现清热活血解毒方药的含药血清可抑制多种银屑病相关细胞的体外增殖，且能够影响角朊细胞内cAMP水平、CaM、 NF-KB基因的表达，而以上因子是银屑病相关信号传导通路：cAMP信号通路、Ca2+-CaMK、NF-KB信号通路的重要组成部分。因此，我们认为"清热活血解毒法"用靶点可能不仅仅是某一条信号传导通路，而是多条信号传导通路组成的信号传导网络。cAMP元件结合蛋白（CREB）是多条银屑病发病相关信号传导通路交织的节点，可以启动下游信号因子瀑布的释放，参与银屑病的发病，因此我们提出新的研究假说CREB及其信号传导网络可能是"清热活血解毒法"调节信号传导网络，治疗银屑病的关键靶点。

银屑病是一种多基因遗传背景下,受环境因素影响的免疫介导下发生的红斑鳞 屑性皮肤疾病，发病率高，严重影响患者身心健康，临床研究显示中医药治疗银屑病是具有 一定优势，然而其治疗的作用机制却有待于深入研究。cAMP 元件结合蛋白 （CREB）是多条银屑病发病相关信号传导通路交织的节点，可以启动下游信号因子瀑布的释 放，参与银屑病的发病。临床及体外研究中我们发现： “清热活血解毒法” 能抑制CREB 的表达并对其相关信号传导蛋白的表达Akt、P38具有抑制作用。因此，我们认为CREB可能是“清热活血解毒法”调节信号传导网络，治疗银屑病的关键靶点。