鸡补体因子C3d增强B淋巴细胞功能信号转导通路的研究
基本信息
结项摘要
补体因子C3d 能够有效地增强与其结合的抗原分子的免疫原性。我们的前期研究证实，鸡补体因子C3d虽然也有此功能，但其基因结构上与其它动物有很大差异。本研究拟将1－2个拷贝串联的C3d基因和禽流感病毒的M2连接，制备含M2-C3d的融合蛋白和DNA疫苗免疫SPF鸡，应用免疫细胞化学染色和流式细胞仪体外检测B细胞增殖；实时定量逆转录聚合酶链反应（Real time RT-PCR）法、染色质免疫沉淀分析（CHIP）等技术检测NF-κB的mRNA水平和表达以及核移位情况；用Western Blotting检测蛋白酪氨酸激酶（PTK）活化、I-κB磷酸化；用荧光分光光度计测定细胞内Ca2+浓度变化；用蛋白结合法测定细胞的环化腺甘酸（CAMP）变化；探讨鸡C3d和鸡B细胞及Raji细胞的表面受体结合及其胞内信号转导通路，从分子和细胞水平阐明鸡补体因子C3d增强B淋巴细胞对抗原免疫应答的作用机理。
项目摘要
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数据更新时间：2023-05-31
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