有效失活大豆抗营养因子特异蛋白酶的筛选

The utilization of soybean proteins is limited by antinutritional factors(ANFs). In the present experiments, we choused the specifical proteinases to inactivate antinutritional factors in soybeans. The study showed that microbiological enzymes can inactivate antinutritional factors in soybeans with deferent degree. Thus the manufactured and nutritional characteristics on soybean protein may be built up in some extent. The growth characteristics of rats are also increased. At the same time the results confirmed there are the specifical proteinase on inactivate antinutritional factors in germinating soybeans. It could be isolated and purified from the soybean of germination. The experiments had validated that the enzymes had the ability to inactivate the antinutritional factors of the soybean. Furthermore β-conglycinin in soybean was purified by salt precipitate and gel filtration on a Sepharise 6B column. Soybean meal was hydrolyzed by Alcalase, Nutrase, AS1 398 and Papain respectively. The result showed there was few difference in the speciality to hydrolyze the allergic protein among these enzymes. The concentration of.β-conglycinin in soybean meal was compared after different degree of.hydrolysis with Alcalase. There was little effect on the quantity of β-conglycinin.when the DH was below 10%. The β-conglycinin in soybean meal was.inactivatesd wholly when the DH reached 25%.

研究大豆在发芽过程中胰，糜蛋白酶抑制因子凝集素和抗原蛋白的变化，以便发现能失活这些抗因子的特异蛋白酶；另外，在已有工作的基础上，从目标微生物中筛选特异降解大豆抗营养因子的蛋白酶，并进行酶学等克隆工程的前期研究。意义在于开辟新的大豆加工途径，既能有效地失活抗营养因子，又不破坏其中的必需养分，成果在饲料和食品加工中价值大。