基于碳点力致荧光变色材料的制备及其生物应用

Carbon dots (Cdots) as a rising material have attracted much attention in the field of biological sensing and imaging. However, the present reported Cdots show poor capabilities of intelligent sensing and target recognition, which has limited its applications in the field of biological sensing and disease diagnosis. . Herein, we propose the fabrication of Cdots based mechanochromism fluorescent materials for biological sensing and diagnosis of thrombosis. The mechanochromism fluorescent Cdots will be formed to improve its intelligent sensing capability by modification in three strategies: surface functional groups, structure, polymer. On this basis, the mechanochromism mechanisms of probes will be investigated by the evaluation of the force sensing efficiency. The obtained mechanochromism fluorescent materials will be further modified with biomarker and applied in thrombosis diagnosis. These probes will provide accurate lesion positioning and avoid signal interference of non-target area through fluorescent color changes induced by pressure, and broaden the applications of Cdots to the field of biological sensing and disease diagnosis.

碳点作为一种新兴材料，已成为生物传感与成像领域的研究热点。然而，现有碳点智能感应能力低、靶向识别效果差，限制了碳点在生物传感及疾病诊断领域中的应用。本项目根据血栓疾病的特点，从表面官能团修饰、结构变化、聚合物包覆三个策略对碳点进行修饰，制备基于碳点的力致荧光变色材料，实现提高碳点智能感应能力的目的。在此基础上，评价探针的力感应能力，阐明碳点荧光变化的机理，并将所得探针进行靶向修饰，应用于血栓疾病诊断。该类探针通过感应压力变化改变荧光颜色，避免探针在非靶标部位沉积所造成的信号干扰，提升成像效果，实现病灶部位准确定位，进而拓展碳点在生物传感与疾病诊断领域中的应用范围。

碳点作为一种新兴纳米材料，具有荧光性质优良﹑抗光漂白能力强﹑毒性低﹑生物相容性好等优点，在材料和分析检测等领域得到广泛关注。本项目围绕构建新型功能化碳点及探索碳点力致响应能力的研究而展开。通过筛选出碳源，并探索出一步合成的方法制备了系列新型荧光碳点。该类碳点可以实现对外界研磨作用力的响应，荧光强度及颜色会发生改变。当遇到酸碱蒸汽可以实现荧光恢复。将该类材料与PMMA进行结合，成功制备出薄膜。该类薄膜仍具有力致响应能力，有望用于防伪、检测应用。此外，通过与纳米粘土杂化材料结合，可以实现力致荧光/磁共振双模态信号的同时变化。通过实验发现，此类力致响应碳点的变化机理主要来源于受力过程中的结构变化以及氢键改变。. 本项目通过赋予碳点力致响应能力，不仅为构建基于碳点智能化检测器提供了技术支持、拓展了碳点的应用范围，同时也为新型碳点的设计、应用提供了思路。所制备的力致响应碳点具有良好的荧光变化效果及可修饰性能，有望在防伪、压力传感检测器等领域得到应用。