基于Butelase 1处理的多肽逻辑门及其癌症靶向治疗应用研究

Traditional single target cancer treatment always suffers from the risk of off-target during the therapeutic process. Hence, the development of high-efficiency molecular recognition technology is of great need to solve off-target problems for the cure of cancers. In this study, a novel peptide logic gate-based molecular recognition strategy was proposed and applied to the targeted cancer therapy. Firstly, a superior biological processor (Butelase 1,a peptide cyclase) was intended to be obtained through the molecular cloning technique. Based on the Butelase 1, a peptide logic sensing platform was attempted to be constructed, hoping it with admirable environmental adaptability and excellent processing property. Then, taking inherent advantage of the peptide logic gate, such as the molecular distinguishing ability and the character of logical processing, an efficient analysis method was put forward for cancer cell recognition. Moreover, the peptide logic gate-based sensing platform was applied to the targeted apoptosis of hepatoma cell. After completion of the project, new molecular recognition techniques and logical sensing methods will be obtained, and new progress in targeted cancer therapies will also be achieved.

高效的分子识别技术对癌症的靶向治疗具有重要意义。针对传统癌症治疗过程中的靶向性问题，申请人提出了基于多肽逻辑门的癌症靶向治疗策略。在前期多肽逻辑门的研究基础上，本项目通过发掘性能卓越的生物处理器（Butelase 1）,用于改善多肽逻辑门的环境适应性、处理效率和和靶向治疗效果。拟采用分子克隆技术获得稳定、高效的多肽环化连接酶Butelase 1，用于多肽逻辑门的构建。并开展多肽逻辑门的生物效应应用研究，获得多肽逻辑门介导细胞凋亡的传感方法，并进一步用于癌细胞的识别和肝癌细胞的靶向治疗。项目完成后将获得新的分子识别技术和逻辑传感方法，同时有望在癌症靶向治疗方面有所突破。

高效的分子识别技术对癌症的靶向治疗具有重要意义。针对传统疾病治疗过程中的靶向性问题，提出了基于多肽逻辑门的癌症靶向治疗策略，通过发掘性能卓越的生物处理器（Butelase 1）,用于改善多肽逻辑门的环境适应性、处理效率和和靶向治疗效果。项目采用分子克隆技术获得稳定、高效的多肽环化连接酶Butelase1，用于多肽逻辑门的构建，开展了多肽逻辑门的生物效应应用研究，获得多肽逻辑门介导细胞凋亡的传感方法，并进一步用于疾病识别和靶向肽筛选。本项目立项以来，在本领域重要学术期刊上发表 SCI 收录论文 3 篇，参加会议两次，申请专利1件，一些重要研究成果发表在Journal of Agricultural and Food Chemistry，被甄选为封面论文，被国内外多家科研媒体报道。