MARVELD1基因调控肝细胞癌介入治疗的机制研究

This research study the correlation of tumor suppressor genes MARVELD1 and transcatheter arterial chemoembolization（TACE） for hepatocellular carcinoma(HCC),the relationship between MARVELD1 gene regulation and the sensitivity of HCC to chemotherapeutic drugs in interventional therapy from the molecular expression, gene regulation and clinical treatmentof multiple perspectives intensively. To determine whether the effect of chemotherapeutic drugs on gene methylation is the mechanism of inhibition of tumor. To explore the promotion of MARVELD1 gene to modulate the malignant phenotype of HCC cells, mechanism of action on the proliferation and growth slower in HCC cells, reveal its molecular basis, to improve our understanding of the role of TACE in the treatment of HCC, to provide a reasonable and effective way for the interventional treatment of HCC. To clarify the mechanism of TACE and to explore a new direction for the treatment of HCC at the molecular level.

针对TACE治疗肝细胞癌作用机制尚未明确的问题，开展抑癌基因MARVELD1调控HCC介入治疗机制的研究。我们前期研究结果示：在HCC肿瘤组织中除基因位点缺失以外，其启动子CpG位点过甲基化是导致HCC细胞该基因表达沉默的重要原因。MARVELD1基因的表达与HCC的发生密切相关，有望为HCC患者临床诊断提供新的有益线索。因此我们提出TACE可能通过促进HCC的MARVELD1基因的表达和功能来治疗HCC的假说。为验证此假说，拟通过探讨TACE对MARVELD1分子表达、基因调控及基因甲基化表达等多个角度的影响，分析MARVELD1基因与TACE治疗相关性，研究MARVELD1基因调控与HCC对化疗药物敏感性关系，揭示抑制基因甲基化药物是否安全有效。为阐明TACE治疗HCC的作用机制及在分子水平治疗HCC提供新思路。