基因修饰的Schwann细胞脑干移植促网状结构损伤修复的研究

he injury of brain stem reticular structure induce high mortality.How to improve the therapeutic efficacy is the difficult and urgent problem of neurosurgens.Schwann cells can clear local necrosis.Neurotrophins can protect the neuron and facilitate the regeneration of axon .Therefore,pcdna3-BDNF and pcdna3- NT-3 were bulid ,and Schwann cells which were transfected with pcdna3-BDNF or pcdna3- NT-3 ex vivo were transplanted into injured reticular formation of rats' brain stem. The result of research indicate that the level of BDNF and NT-3 which were secreted by transgene SW cells were improved significantly; transgene SW can protect neural cells effectively and facilitate the regeneration of axon.This provid a new way in molecular level to treat brain stem injury.

脑干网状结构损伤重残率、病死率高，近年一直是神经外科界的一个研究热点，也是一难点，传统的治疗手段难以奏效。Schwann细胞可吞噬局部坏死细胞及组织、分泌神经营养蜃樱佣兄谏窬⑸窬嵬坏脑偕谏窬缃峁怪亟ê凸δ芑指础１究翁饽獠捎昧匣蜃荆玀HC、BDNF、NT-3等分子的编码基因导入猪的Schwann细胞，旨在提高熘窒赴浦埠蟮拇婊盥屎推浞置谏窬蜃拥哪芰Α＝蛐奘魏蟮