在蒙药方剂中入药动物心脏的科学依据及质量标准研究

The research of Mongolian herbal medicine is the basis for the development of Mongolian pharmacy.Animal medicine is One of characteristics in Mongolian pharmacy. Animal hearts are often used in the Mongolian prescription using to treat "XinHeYi". Thinking the yak heart is the best choice, but it is only " hypothesis "； The Preparation of Mongolian Prescription has peculiar smell and can be contaminated easily when Yak heart was used in the form of dried Powder；Different kinds of animal heart are using freely；Lack of quality standard. The project shall study the efficacy of the Preparation of Mongolian Prescription when using dried yak heart, freeze-dried powder as well as lack of animal hearts. Through this way, determine the action of yak heart in prescription as well as whether the freeze-dried powder can be used as an alternative；Through comparing the efficacy of different preparations of Mongolian prescription which were prepared by adding freeze-dried powder of different animal hearts, clarifying the scientific basis about yak heart being the best to treat "XinHeYi" , confirming " hypothesis"；Through the cell test, immunological tests and electrophoresis test, further clarifying the conclusion of above animal experimental results and confirming " hypothesis"; Through the component analysis, in chemical composition level, further clarifying the conclusion of above animal experimental results and confirming " hypothesis", and also providing component basis and method basis for the research of quality standard; Unifying animal hearts to " yak heart ", separately researching the quality standards of yak heart dried powder and freeze-dried powder. The project has important significance for the Standardization of characteristic Mongolian Medicine and its improvement of technology content and inner quality.

蒙药材研究是蒙药学发展的基础。使用动物药是蒙药特色之一。蒙医治疗"心赫依"病方剂中常有动物心脏入药，认为牦牛心是最佳选择，但这是"假说"；牦牛心风干后入药，方剂具异味且易染菌；多种动物心脏在随意使用；动物心脏药材缺少质量标准。本项目对入药风干牦牛心、冻干粉以及缺少该味药的蒙成药做动物药效实验，阐明牦牛心的作用及可以用冻干粉替代的依据；通过对不同动物心脏入药的蒙成药做动物药效研究，阐明牦牛心入药治疗"心赫依"病最佳的科学依据，证实"假说"；通过细胞试验、免疫学和电泳试验，进一步阐明动物实验结果的结论，并证实"假说"；通过成分分析，在内在物质成分层面上，进一步阐明体内药效学试验结果得出的结论，并证实"假说",也为质量标准的研究提供成分依据和方法依据；将动物心脏用药统一到"牦牛心"，对牦牛心冻干粉和风干粉进行质量标准研究。该项目对蒙医特色药材规范化、对提高其科技含量和内在质量均具重要意义。

蒙药材研究是蒙药学发展的基础。使用动物药是蒙医用药特色之一。蒙医治疗“心赫依”病方剂中常有动物心脏入药，认为牦牛心是最佳选择，但这是“假说”；牦牛心风干后入药，方剂具异味且易染菌；多种动物心脏在随意替代使用；动物心脏药材缺少质量标准。本项目对肉豆蔻-8散进行了对实验动物心脏保护作用的研究；对入药风干牦牛心、冻干品以及缺少该味药的肉豆蔻-8散进行了药效学实验研究；对入药不同动物心脏的肉豆蔻-8散进行了药效学对比研究。结果显示，肉豆蔻-8散具有较好的实验动物心脏保护作用；动物心脏配伍到组方中是必要的；含牦牛心的肉豆蔻八味散在对实验动物心脏保护作用方面较含有其他动物心脏的肉豆蔻-8散更好。本项目进一步在药代动力学、体外细胞试验、PCR试验、对ATP能量代谢影响等层面开展研究，其结果均显示不同动物心脏之间的差异和牦牛心具有的优势。该项目对蒙医特色药材使用的规范化研究以及特色蒙药材科学内涵的揭示具有重要意义。项目对依托方剂“肉豆蔻-8散”的质量标准进行研究，其标准被收入内蒙古自治区地方标准中；对牦牛心冻干品质量标准进行了研究，牦牛心冻干品质量标准草案及起草说明见附件。本项目发表科研论文11篇，其中中文核心期刊论文8篇；培养博士2人，硕士研究生7人，本科生9人。