基于高分辨红外光谱成像及ATR空心波导红外光谱学的骨关节炎基础研究

As a kind of intractable diseases, osteoarthritis (OA) influences people's life and health and is still difficult to effectively cure in clinical. Research on the pathogenesis and treatment technology of OA is the main direction of this field, the pathological exploration of OA is the key for effective treatment. This proposal presents OA new study method, (1) using Fourier transform infrared (FTIR) spectroscopic imaging high spatial resolution (6.25 μ m/pixel) combined chemometrics, to investigate the principal component concentration and structure information of articular cartilage from the surface to the inside, (2) using attenuated total reflection - hollow waveguide FTIR spectroscopy and chemometrics, to determine the incidence locations of in vitro OA cartilage surface and OA stage. Then the above two kinds of combined infrared technique will be applied to carry out basic research of OA pathomechanism. It includes to quantify the cartilage (principal component concentration and structure) degeneration in different parts of cartilage at different OA stage; to analyze principal component concentration distribution, fine structure arrangement in cartilage, and function relation between them; to reveal the the locations at which the OA occurs easily, the rules of OA occurrence and development. The results of the present project not only have important reference value for the prevention, diagnosis and treatment of OA, but also lay the foundation for clinical application.

骨关节炎(OA)是一种影响人们生活与健康的顽固性疾病，临床仍然难以有效治愈。OA的发病机理与诊疗技术研究是该领域的主要发展方向，其中OA的病理基础探索是进行有效治疗的关键。本课题首先提出了新的OA研究方法，（1）采用高空间分辨率（6.25μm/pixel）傅立叶变换红外(FTIR)光谱学成像结合化学计量学，切片研究关节软骨的由表及里主成分浓度与结构信息，（2）采用衰减全反射(ATR)－空心光波导－FTIR光谱学和化学计量学联用技术判定OA离体软骨表面发病部位，界定OA分期。然后利用上述两种红外联用技术方法开展OA病理基础研究，定量不同OA分期不同部位的软骨(主成分浓度和结构)退变程度，分析软骨主成分精细结构排列、浓度分布以及它们之间的功能关系，揭示OA易发病位置、发生和发展规律。本项目研究结果对OA的预防、诊断和治疗有重要的参考价值，为临床医学应用打下基础。

骨关节炎(OA)是一种影响人们生活与健康的顽固性疾病，临床仍然难以有效治愈。OA 的发病机理与诊疗技术研究是该领域的主要发展方向，其中OA 的病理基础探索是进行有效治疗的关键。本课题首先建立了新的OA 研究方法，（1）采用高空间分辨率（6.25μm/pixel）傅立叶变换红外(FTIR)光谱学成像结合化学计量学，获得了关节软骨的由表及里主成分浓度与结构信息，（2）设计、建立、并采用衰减全反射(ATR)－空心光波导－FTIR 光谱学联用技术进行了OA软骨离体原位探测，并和化学计量学联用判定OA软骨表面发病部位 （半月板未覆盖区），判定OA 分类(5组，87%准确率)，结合病理分析实现了光谱定量原位组织的OA分期。利用上述两种红外联用技术方法开展OA 病理基础研究，定量研究了不同OA 分期不同部位的软骨退变程度，获得了软骨主成分结构、浓度分布以及它们之间的功能关系，揭示了OA 易发病位置、发生和发展规律。本项目研究结果有利于更准确地实现在体原位判断OA的发病程度，对OA 的预防、诊断和治疗有重要的参考价值和指导意义，为相关理论普及和临床应用奠定了一定的技术和理论基础。