Endoglin基因修饰肿瘤/DC杂交细胞诱生靶向特异性抗人肺癌CTL疫苗的研究
基本信息
结项摘要
Endoglin(EDG)是肿瘤新生血管内皮细胞膜表面蛋白，是肿瘤血管生成的标志性分子之一。树突状细胞(DC)是体内抗原提呈功能最强的抗原提呈细胞。本研究拟在完成省自然科学基金子课题"抗肺癌DC疫苗研究"和国家自然科学基金项目的子课题"EndoglinDNA疫苗诱导抗癌免疫反应"的基础上，以体内癌细胞和肿瘤新生血管内皮细胞为直接目标，依据肿瘤抗原提呈给T细胞的机理，将转染鼠EDG表达基因的原代培养的肺癌患者癌细胞与患者的DC融合成杂交细胞，该细胞与患者的T细胞混合培养诱生特异性CTL，完成体外细胞毒实验。用原代培养的人肺癌细胞与重建肺癌病人免疫系统的SCID鼠建立肿瘤动物模型，将转染鼠EDG基因的荷瘤鼠癌细胞和荷瘤鼠DC融合成杂交细胞后，再与荷瘤鼠T细胞混合培养，诱生扩增的CTL回输至荷人肺癌SCID鼠体内，研究其对体内癌细胞和肿瘤血管内皮细胞直接产生"双重"细胞毒作用所诱导的抗癌效应。
项目摘要
项目成果
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数据更新时间：2023-05-31
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