散寒通络法干预类风湿性关节炎滑膜巨噬细胞表达细胞因子网络机理研究

Rheumatoid arthritis (RA) is equivalent to "Arthralgia-Syndrome" in traditional Chinese medicine which is caused by more factors and its pathogenesis is complicated.Mr. Bai Qingzuo who is one of four late famous doctors in my province thinks that cold retained in meridinas is the key link in causing RA and had achieved very good curative effect in treating RA by dispelling cold and activating collaterals.On the basis of previous research,in this subject we put forward that the treatment of dispelling cold and activating collaterals for RA may be through the intervention of synovial macrophage related cytokines network.therefore,We elect Mr. BaiQingZuo 's Empirical prescription-FengShiNing capsule and apply the modern experimental technology to observe the effect of the treatment of dispelling cold and activating collaterals on experimental RA Zealand white rabbit including general conditions,pathological morphology of synovial tissue,the expression of IL-1、TNF-a、IL-4、IL-6、IL-8、IL-10、IL-12、IL-15、IL-17、IL-18 in serum and joint synovial fluid，the expression of MMP-3、 MMP-9、CyPA、VEGF in synovial tissue and so on.through the statistical analysis of the correlation between various cytokines, clarify the mechanism and key links action of FengShiNing Capsule in treating rheumatoid arthritis and provide a scientific basis and effective prescription for the clinical application of dispelling cold and activating collaterals therapy for rheumatoid arthritis。

类风湿性关节炎（RA）属于中医"痹证"范畴，其发病多端，病机复杂。我省已故四大名老中医之一白清佐先生认为寒凝经脉是RA发病的关键环节，并采用散寒通络法治疗RA取得了很好的疗效。本课题在前期研究的基础上，提出散寒通络法治疗RA可能是通过干预滑膜巨噬细胞相关细胞因子网络而起效，故拟选用白清佐先生的经验处方风湿宁胶囊，应用现代实验技术，观察散寒通络法对实验性RA新西兰大白兔的一般情况，滑膜组织病理形态，血清及关节滑膜液IL-1、TNF-a、IL-4、IL-6、IL-8、IL-10、IL-12、IL-15、IL-17、IL-18，滑膜组织MMP-3、MMP-9、CyPA、VEGF等因子的影响。通过统计分析各细胞因子间的相关性，以期阐明风湿宁胶囊治疗类风湿性关节炎的作用机理和关键环节，为临床应用散寒通络法治疗类风湿性关节炎提供科学依据和有效方药。