从胰岛素信号转导通路与脂质代谢途径研究芪茵颗粒改善NAFLD的作用机制

Xinjiang prevalence of NAFLD 34.09%, higher than the national average. This preliminary study found that local NAFLD subjects syndromes have regional characteristics. "Qi Yin particles" Hospital of Xinjiang Uygur Autonomous Region hospital agreements prescription, to comply with the Xinjiang regional characteristics of the compound, more than 20 years of clinical use. Pharmacodynamic study results show that the particles can relieve Qi Yin NAFLD patients with liver function, improve patient body mass index (BMI), serum ALT, TG, liver B ultrasonic indicators. But the mechanism is unclear. NAFLD-related issues through the establishment of the rat and cell model, using Real-time PCR, Western blot, immunohistochemistry, and streaming pathological modern pharmacology, pharmacodynamics experiments with cells from two levels of the experiment, explore treatment of NAFLD Qi Yin mechanisms and molecular targets. Implementation of this project is expected to build Xinjiang Chinese medicine prevention, treatment and research NAFLD comprehensive technology platform to enhance the prevention and treatment of NAFLD Xinjiang medical standards.

新疆地区NAFLD的患病率为34.09%，高于全国平均水平。本课题前期研究发现当地NAFLD中医证候有地域性特征。"芪茵颗粒"基于非酒精性脂肪肝"肝脾肾同调，痰瘀滞同治"的创新理论组方，是新疆维吾尔自治区中医院院内协定处方，为符合新疆地域特点的复方制剂，使用20余年。临床研究结果表明芪茵颗粒能明显缓解NAFLD患者肝功能，改善患者体重指数(BMI)、血清ALT、TG、肝脏B超等指标。但作用机制尚不清楚。本课题通过 建立NAFLD相关大鼠及细胞模型，采用Real-time PCR、Western blot、免疫组化、病理形态学及流式等现代药理学方法，从整体动物药效学实验与离体细胞实验两个层次，探讨芪茵颗粒治疗NAFLD作用机制及分子靶点。本课题的实施有助建立新疆地区中医药预防、治疗及研究NAFLD的综合性技术平台，全面提升新疆地区防治NAFLD的医疗水平。

新疆地区NAFLD的患病率为34.09%，高于全国平均水平。本课题前期研究发现当地NAFLD中医证候有地域性特征。“芪茵颗粒”基于非酒精性脂肪肝“肝脾肾同调，痰瘀滞同治”的创新理论组方，是新疆维吾尔自治区中医院院内协定处方，为符合新疆地域特点的复方制剂，使用20余年。临床研究结果表明芪茵颗粒能明显缓解NAFLD患者肝功能，改善患者体重指数(BMI)、血清ALT、TG、肝脏B超等指标。但作用机制尚不清楚。本课题通过 建立NAFLD相关大鼠及细胞模型，采用Real-time PCR、Western blot、免疫组化、病理形态学及流式等现代药理学方法，从整体动物药效学实验与离体细胞实验两个层次，探讨芪茵颗粒治疗NAFLD作用机制及分子靶点。本课题的实施有助建立新疆地区中医药预防、治疗及研究NAFLD的综合性技术平台，全面提升新疆地区防治NAFLD的医疗水平。