平滑肌前体细胞移植介导内皮祖细胞基因甲基化作用早期干预动脉硬化性脑梗塞的分子机制研究

Atherosclerosis is the main reason of cerebral infarction. The progression of smooth muscle progenitor cell(SPCs) and endothecial progenitor cell(EPCs) bring a new hope for the intervention of atherosclerosis. But the molecular mechanism is unknown. This study uses green fluorescent protein (GFP) as reporter gene,bulids a system of SPCs and EPCs labeled EGFP with the lentivival vector, detects EPCs gene methylation with different concentration of Hcy in vitro,establishes an atherosclerosis mouse model,uses gene chip technology methylation specific PCR and Real-time PCR technology,disscusses the molecular of early intervention of SPCs transplantation mediating EPCs on atherosclerosis ,reveals the progress of intervention between stem cell and atherosclerosis and supplys theoretical basis for clinical ues of stem cell in atherosclerosis.

动脉粥样硬化血栓性脑梗死是脑梗塞中的主要原因。平滑肌祖细胞（SPCs）和内皮祖细胞（EPCs）的发现为干预动脉粥样硬化性脑梗塞带来了新的方法，但目前对其移植治疗动脉硬化血栓性脑梗塞的分子机制仍不清楚。本研究拟以绿色荧光蛋白（GFP）作为报告基因，慢病毒载体系统构建GFP标记的SPCs和EPCs，并在体外对不同浓度的 同型半胱氨基酸（Hcy）对EPCs基因甲基化作用进行检测，同时通过建立动脉粥样硬化血栓性脑梗死的小鼠模型，利用基因芯片技术、甲基特异性PCR技术和Real-time PCR技术等，从分子水平上探讨SPCs移植介导的内源性内皮祖细胞对动脉硬化性脑梗死早期干预的机制，为了解干细胞移植干预动脉硬化的发生发展，解决这一重大影响人类健康的疾病和临床干细胞治疗动脉硬化脑梗塞提供理论基础。