环境与遗传交互作用在职业紧张所致高血压、心理障碍疾病的队列研究

Occupational stress has become among the most crucial occupational health issues internationally. Therefore the relationship between occupational stress and the outcome of health cost should be studied thoroughly and clearly for the prevention of occupational stress.It has been explicitly illustrated that specific environments can lead to an elevated possibility of occupational stress, causing decline in physical and mental health status and thus occurrenceof disease. However, since most previous studies only involve cross-sectional studies and case-control studies, etiological evidence remains insufficient. Thus in our research, the method of case cohort study and ambispective cohort study are used to confirm the epidemiology and time trend of hypertension and mental disorder in people who work in such special environment as alpine plateau and arid desert, and to further verify the etiological relationship between environmental stress factors and hypertension as well as psychological disorder. In the meanwhile, it is predicted that corresponding susceptibility genes in hypertension and psychological disorder can be selected and confirmed. The correlation of epigenetics characteristics with psychological disorder and hypertension can be explored. The interaction of environmental factors, genetic factors, epigenetics marks corresponding to hypertension and psychological disorder can be clarified. Based on the research above, a predictive model for the risk of hypertension and psychological disorder can be built. So the scientific basis on the detection and prevention of hypertension and psychological disorder can be provided.

职业紧张已经成为当今重要的世界性职业卫生问题之一，研究职业紧张与健康损害结局关系对于职业紧张的防治具有重要的意义。有研究表明特殊自然环境会进一步加剧职业紧张的出现，引起生理心理健康状况明显下降，继而导致疾病的发生。但由于已有的研究都是建立在横断面或病例对照研究的基础上，缺乏有力的病因学证据。因此本项目在课题组前期调查基础之上，预期通过双向性队列研究和病例队列研究明确在新疆高原高寒和干旱沙漠特殊环境下作业人群中高血压和心理障碍的流行现况及其时间趋势，进一步证实环境紧张因素与高血压和心理障碍之间的病因关系，筛选和验证高血压、心理障碍的相关易感基因；探讨表观遗传学特征与高血压、心理障碍之间的关联；明确与高血压、心理障碍发病有关的环境因素、遗传因素、表观遗传标志之间的相互作用。在此基础上构建高血压、心理障碍风险预测模型，为职业人群高血压和心理障碍的早期检测和预防提供科学依据。

职业紧张已经成为当今重要的世界性职业卫生问题之一，研究职业紧张与健康损害结局关系对于职业紧张的防治具有重要的意义。有研究表明特殊自然环境会进一步加剧职业紧张的出现，引起生理心理健康状况明显下降，继而导致疾病的发生本次研究发现特殊环境作业人群职业紧张得分高于全国常模，随着职业紧张程度的增加，付出-回报失衡感越重，焦虑抑郁的检出率都有所增加，职业紧张和付出-回报失衡是特殊环境作业人群高血压和心理障碍发病的主要危险因素；职业紧张水平升高组和个体紧张反应升高组发生高血压、心理障碍的风险更大。职业紧张和GR BclⅠ的交互作用可以增加高血压的发病风险，T102C、A-1438G与职业紧张的交互作用可以增加心理障碍的发病风险。450K甲基化芯片筛选出高度职业紧张组存在较多的低甲基化位点，低度职业紧张组高甲基化位点较多，不同的染色体所含差异甲基化位点不同，ISLANDInd区域差异甲基化基因最多，高血压与职业紧张在全基因组层面上存在甲基化表达谱的差异。心理障碍患者高度职业紧张组和低度职业紧张组CpG1及CpG7之间比较，差异有统计学意义（P＜0.05） 通过不断地调整和优化，所构建的人工神经网络模型能够较好的预测高血压疾病，其预测准确率高达85.8%，假阳性率仅为2.2%。通过控制职业紧张水平，可以降低人群高血压、心理障碍的发病率，具有一定的公共卫生意义。