1，3-丁二烯诱导人淋巴细胞HPRT基因突变及其修复的研究

1,3-Butadiene (BD) is a kind of important industrial material, that is widely used in the production of resins and plastics such as butadiene rubber and polychloroprene. The toxicological effects of butadiene have been given more attention. In order to get details about cloning efficiency, mutation frequency, the spectra, mechanism and repair of HPRT gene mutation induced by butadiene, blood lymphocytes of BD-exposed workers and control subjects were examined by methods of cell clone culture, two-way screening count, multiplex PCR amplification, electrophoresis and transgenic techniques. The number for BD-exposed workers and control subjects was 74 and 157, respectively. It was discovered that mutation frequency of BD-exposed workers was higher than control subjects', but not significantly (P>0.05). The percentage of deletion mutation in BD-exposed subjects was bigger than that in control subjects significantly (P<0.05), in particular multiplex exons often deleted at the same time. Allied results were got in vitro tests, too. It suggested that BD was liable to cause bigger changes in genetic structure, which showed health of BD-exposed workers could not be protected in present country healthy standard (100mg/m3). This is the first time that heredity affect of BD-exposed workers were investigated in system in our country. The research could give some help to afresh country BD healthy standard, to build the international database of HPRT gene mutation and to study mechanism of genetic disease with HPRT lack.

抽屉，3-丁二烯作业人员静脉血，进行淋巴细胞培养，在含6-TG培养基中筛选HPRT突变体，计算突变体频率；对阳性克隆HPRT基因进行PCR扩增、电泳及测序。同时用磷酸钙沉淀法和缏龀宸ㄏ騂PRT细胞转移人正常的HPRT，筛选HPRT+细胞，并测定HPRT活性。最终确定1，3-《┑姆肿油槐淦住⑼槐浠砑靶薷吹目赡苄浴Ｎ匦轮贫ㄆ湮郎曜家约爸耙捣阑ぬ峁├砺垡谰荨