嵌合RNA-(SPARC-LOC)与亲本基因的共轭效应及其调控猪骨骼肌发育的分子机制

Improving the meat production, which depends on muscle growth and development，is the main goal of the pig breeding. Chimeric mRNAs are expected to increase the diversity and complexity of transcriptomes and proteomes through chimeric sequence segments or altered regulation. However, function of chimeric mRNAs in muscle development is unclear. Chimeric mRNAs can regulate the activities through signaling pathway together with its pre-mRNA. Chimeric RNA-(SPARC-LOC) was identified as a fusion gene SPARC-LOC100737020 in porcine skeletal muscle. It was relatively highly expressed in muscles and 2.4-fold in fetal muscles than in adult muscles. Previsous research found SPARC played an important role in regulating the proliferation and differentiation. So we put forward a scientific hypothesis that “there is conjugate effect between chimeric RNA-(SPARC-LOC) and SPARC which can affect the growth and development through signaling pathway in porcine skeletal muscle”. This project was designed to study the structure and formation as well as the function of chimeric RNA-(SPARC-LOC). Conjugate effect between chimeric RNA-(SPARC-LOC) and SPARC will be analyzed . The regulation mechanisms of this chimeras and its pre-mRNA through PI3K/Akt signaling pathway in porcine skeletal muscle development will be revealled, which will provide a new perspective and solution in regulation of meat production.

产肉性状是猪遗传育种工作的主要目标，关键取决于骨骼肌的生长发育。嵌合RNA具有重要的生物学功能，与其亲本基因可发生共轭效应，并介导相关信号通路调节机体生命活动，但在肌肉发育中的调控作用及机制尚不清晰。本课题组率先在猪骨骼肌中发现嵌合RNA-(SPARC-LOC)，鉴定其在猪骨骼肌组织中特异性高表达，在生长发育过程中呈下调表达模式。该嵌合RNA的亲本基因SPARC可促进骨骼肌发育与再生，与细胞增殖、分化及胚胎发育等生命活动密切相关。据此我们认为：嵌合RNA-(SPARC-LOC)与SPARC可能存在共轭效应，通过介导信号通路调节骨骼肌发育。本研究拟从分子、细胞和个体水平对嵌合RNA-(SPARC-LOC)开展深入研究，阐明其分子特征、编辑方式和生物学功能，解析嵌合RNA与其亲本基因介导相关通路调控骨骼肌发育的机制，明确其在骨骼肌发育中的遗传效应，为理解骨骼肌生长发育分子机制提供新的理论基础。

为了加强猪种质资源创新，培育出性能优良的种猪，解析猪产肉性状形成的分子机制，有效挖掘利用中国地方猪种质特性和深入实现种质创新。.本项目通过对大白、梅山、藏猪和五指山小型猪近交系等不同品种不同生长阶段的猪转录谱分析，丰富了猪嵌合 RNA 数据库；完善了多品种猪转录谱数据库、比较转录谱数据库，获得软件著作权 1 项；优化了猪原代肌卫星细胞、猪原代前脂肪细胞的分离、培养体系，获得了可稳定传代的大白、藏猪、梅山猪的原代肌卫星细胞、原代前脂肪细胞；对目的基因嵌合RNA-(SPARC-LOC)的功能、机制进行了系统的研究。对嵌合RNA-(SPARC-LOC)的SNP位点分型，并在日照大白猪群体内完成了与重要经济性状的关联研究。.在 Scientific Reports、Journal of Integrative Agriculture，Oncotarget，Appl Biochem Biotechno等国际刊物发表 SCI 论文4篇，其中：影响因子 5 以上的2篇；在国内核心期刊《农业生物技术学报》发表中文论文1篇；申报国家发明专利1项；获得软件著作权 1 项；协助培养博士后1名,博士研究生1名，硕士研究生1名。