胸腺神经内分泌细胞的研究

Thymic neuroendocrine cells were a group of special diffused secreting cells (APUD cells) in snake thymus( Li and Wang, Science in China(B),1994), under the support of Chinese Nature Science Fundation we have studied the charachteristics of this group of cell from ontogeny to phylogeny with immunocytochemical, in situ hybridization, microstructural and ultrastructural methods. The results show as follows: as a group of diffused cell with different types, the thymic neuroendocrine cells are as same as the neuroendocrine cells in gut and respiratory tract in cellular structure and the synthesizing and secreting hormones. The cells located as closed and open types secret their hormones by the way as paracrine and autocrine and regulate the function of thymic microenvironment and tdevelopment of T cells. The thymic neuroendocrine cells appear at the late stage of thymic development in snake and maybe have the same stem cell with thymic epithelial cells. From the experiment of the relationship of sex hormone with these cells, it also show that these cells can regulate other targets as endocrine cells. These results confirmed that thymus is a neuroendocrine cells in non-mammal vertebrates and play a key path between neuroendocrine and immune systems.. On the other side, the degeneration of thymus is a important and unclear point in aging. We did the rejuvenation of degenerative thymus in vivo and in vitro. The experiment show it is a possible to rejuvenate the degenerating thymus.

以爪蟾、蛇、鸡和大鼠为研究对象，从个体发育和系统演化的角度，应用免疫细胞化学多重染色、免疫电镜、原位杂交和细胞培养等技术，研究胸腺神经内分泌细胞的发育分化、细胞类型、激素共存以及对胸腺微环境和T淋巴细胞发育分化的调节作用，为探讨胸腺在神经内置诿庖咄缰械牡匚坏於ɑ。鲜度死嘈叵偕窬诜置谙赴鎏峁┎慰肌