基于纳米多孔金属膜增敏的SPR-荧光双模态传感技术研究

Nanoporous-metal-film-Enhanced Surface Plasmon Resonance-Fluorescence detection is a novel sensing technology , with the advantages of high sensitivity , high specificity, simple preparation, low cost and no radiation. The basic principles are the interactions of evanescent field and biomolecular, and fluorescence reaction of enzyme-substrate.The nanoporous metal films are used as the sensitive layer of the chips, combining the advantages of field enhancement and large number of the binding sites , making the sensor chips having the characteristics of propagating surface plasmon resonance, localized surface plasmon resonance and the large surface area, and then can on-line monitoring the tumor markers in situ. The project is aimed for the high sensitivity and high specificity detection of tumor marker, to construct a double modes analysis systems based on nanoporous-metal-film-enhanced SPR and fluorescence detection, the main research contents are: (1) the mechanism of nanoporous-metal-film-enhanced SPR-fluorescence sensing system; (2) the optimization design of sensor chip structure and the regulate methods of nanoporous films parameter ; (3) surface modification and functionalization of the sensor chip; (4) construct the dual signal analysis modeling. The project is to provide new methods and new ideas for early screening and evaluation of cancer.

纳米多孔金属膜增敏的SPR(Surface Plasmon Resonance)-荧光检测是一种新颖的传感技术，具有灵敏度高、特异性好、制备简单、成本低廉和无辐射等优点。它以消逝场-生物分子相互作用及酶-底物荧光反应为基本原理。采用纳米多孔金属膜作为SPR-荧光检测芯片的敏感层，利用纳米多孔金属膜的场增强和多结合位点的优势，使其同时兼具传播等离子体共振、局域等离子体共振及大比表面积三重功效，可对肿瘤标志物进行原位在线监测。本项目针对肿瘤标志物进行高灵敏和高特异性检测，构建一种基于纳米多孔金属膜增敏的SPR-荧光检测双模态关联分析方法，主要研究：(1)纳米多孔金属膜增敏的SPR-荧光传感系统敏感机理；(2)传感芯片结构优化设计与参数调控方法；(3)传感芯片表面功能化处理；(4)SPR-荧光双信号关联分析建模。本项目的顺利开展以期能够为癌症的早期筛查和评估提供新方法和新思路。

纳米多孔金属膜增敏的SPR(Surface Plasmon Resonance)-荧光检测是一种新颖的传感技术， 具有灵敏度高、特异性好、制备简单、成本低廉和无辐射等优点。它以消逝场-生物分子相互 作用及酶-底物荧光反应为基本原理。采用纳米多孔金属膜作为SPR-荧光检测芯片的敏感层， 利用纳米多孔金属膜的场增强和多结合位点的优势，使其同时兼具传播等离子体共振、局域等 离子体共振及大比表面积三重功效，可对肿瘤标志物等生物大分子以及生化小分子进行原位在线监测。课题经过仿真验证，确定了敏感芯片的最佳厚度参数是60nm。课题利用纳米多孔金膜对生化小分子双酚A进行了高灵敏的测试，检测下限达到1 nmol∙L−1。课题利用常规金膜SPR芯片实现多种分子的多孔自组装研究，得到有效的动力学规律曲线。课题利用双亲脂质体包裹针对乳腺癌细胞的诊疗一体化载药纳米粒子，并且通过荧光实现准确定位。