电化学制备聚多巴胺复合薄膜在牙种植体治疗中的应用研究

Up to now, there are still some defects in the methods introduced in bioactive molecule, which affect achievement ratio of dental implantation. Our reseasch focus on surface modification of dental implant with polydopamine composite films prepared by electropolymerization (EP). Polydopamine composite films was designed and loaded bioactive molecule, organic/inorganic nanoparticles and functional organic/polymer molecule to build bifunctional antibacterial/bioactive implant coatings. This new type of dental implantation can enhance osseointegration and inhibit the growth of bacteria effectively. Dental implantation with different surface morphology were produced to study effects of surface microstructure on osseointegration, determine relationship between surface microstructure and proliferation and differentiation of osteoblasts, optimize synergy effect of surface antibacterial ability and promoting osteogenesis. This study provide not only new functions for dental implant but also a new way of thinking about surface modification of dental implant and drug sustained release of bioactive molecule and reliable experiments basis for clinical oral planting treatment.

目前的种植体表面引入生物活性分子的方法仍有缺陷，影响牙种植治疗的成功率。申请人拟通过电化学方法制备的钛/聚多巴胺作为种植体基底模型，构建有机小分子、无机纳米粒子、生物活性大分子功能性复合薄膜，得到抑菌/生物活性双功能种植体。这种种植体将有效增加与骨的结合和抑制细菌滋生。通过调节薄膜表面形貌，研究种植体表面微结构对于种植体骨整合能力的影响，明确种植体表面微纳米结构与成骨细胞增殖和分化的关系，优化种植体表面抑菌与促进成骨的协同治疗效果。本项目将赋予种植体表面的新结构和新功能同时，为其表面改性及活性生物分子的缓释方式提供了新的思路,为复合涂层种植体应用于临床口腔种植治疗提供实验依据。

骨结合能力及种植体周围炎是影响钛基牙种植体治疗成功率的重要因素。因此，种植体的表面修饰备受关注。我们通过电化学聚合制备聚多巴胺复合薄膜修饰的种植体并将生物活性分子，有机/无机纳米粒子复合在聚多巴胺薄膜上，构建抑菌/生物活性双功能种植体。结合现代分析技术，如扫描电子显微镜和原子力显微镜等确认了具有不同微纳米结构形貌的银复合种植体表面的制备。以此为基础，将人骨重组蛋白负载在复合薄膜上，构建具有抑菌/生物活性双功能种植体。体外和体内实验表明负载人骨重组蛋白的复合薄膜有优异的抑菌和促成骨效果，并有作为牙种植体治疗的应用潜质。