水稻双子房突变相关基因的定位与克隆

Rice is one of the most important crops of the world and is being studied as a model plant for molecular genetic analysis. The yield and quality of rice,that is the hard-core of economic value, is controlled directly by the development of flora organs. Molecular cloning of genes that regulate floral organ development has been made an explosion of information about genetic and molecular mechanisms of flower development in higher plants. Mutant is the good material for cloning gene and understanding the developmental control mechanisms in the agronomically important flower of rice. In this project, a twin-ovary mutant, which is may relative to the activity of transposable element and is controlled by a single recessive gene, was studied through screening different DNA fragments between the wild-type and the mutant. The gene has been located on the rice molecular map, which is useful for the positional cloning of the gene.

颖花发育直接控制着稻米产量和品质，是水稻的经济价值核心。突变体是克隆基因、研究基因功能的关键材料。本项目利用与逆转座子的活动有关的、受单隐性基因控制的水稻双子房突变体，定位突变性状基因，筛选近等基因系在表达水平和基因组DNA上的差异，通过差异锹椒寺】刂仆槐湫宰吹南喙鼗颍沂舅居被ㄆ鞴俚姆⑸频於ɑ?.