光活性DNA切割剂的研究

The synthetic small molecules that can irreversibly and selectively modify nucleic acid have attracted considerable attention in chemistry and biology. Photoinduced DNA cleaving agents are especially convenient since they are inactive until activated by light. In this research, three kinds of novel photoinduced DNA cleaving agents were synthesized in solution. Its ability of DNA cleaving was investigated with Supercoiled DNA (pBluescript SK) under UV irradiation. The DNA cleaving activities of these novel agents are influenced dramatically by the structure of the DNA binding moiety and the concentration of the cleaving agents, and the cleaving activity was enhanced by the hairpin polyamide. The present study has provided a useful method for the synthesis of the novel DNA cleaving agents. They will allow us to probe deeper into the biological phenomena of interactions between small molecules and DNA, making the cleaving agents a very useful investigation tool in chemical biology and gene-selection drug.

实现小分子与DNA特定碱基序列的识别和切割，调控基因，是生命科学研究领域关注的焦点弧１究翁饨恤驶氐淄橛刖哂邢赴腹缘耐氛胱垂丫埘０吩咏唬铣删哂卸訢NA的碱基序列特异性识别的光活性切割剂，为设计新的基因选择性药物提供先导化合物及理论依据。本项目对生命过程中DNA与小分子间相互作用的研究具有重要大学术意义。