一株海洋细菌利用褐藻胶的代谢途径及关键酶研究

As one of the most abundant marine polysaccharide, alginic acid has broad application prospects in the field of bio-fuels, medicine, food et.al. Alginic acid degrading bacterium can degrade alginic acid into monosaccharide for bacterium growth. However, the alginic acid metabolic pathway and mechanism of the bacteria is still not clear. In this object，the conventional microbiological culture,NMR, HPLC, MS, LC-MS and other modern biochemical analysis method,combined with molecular biological research methods are adopted to investigate the metabolic pathways and mechanisms of the alginic acid in a new species of marine bacteria, BP-2, which has been identified and the complete genome was sequenced in our laboratory. The purpose of the objects is to define the key gene which involved in the metabolic of alginic acid, explore the expression of alginate lyase in bacteria, the mechanism of uptake and metaolic guluronic acid or mannuronic acid at the molecular level, and elucidate the metabolic pathways and the metabolic mechanism of alginic acid in BP-2 cells. This study would provide a theoretical basis for building different alginic acid fermentation strain and converting seaweed to bio-ethanol efficiently.

褐藻胶，又称海藻酸，是最丰富的海洋生物多糖，在生物质能源、医药、食品等领域具有广阔的前景。海藻酸降解菌可以将海藻酸降解为海藻酸单糖供菌体生长发育需要，然而，海藻酸降解菌的海藻酸代谢途径及其机制还不完全清楚。本课题应用分子生物学研究手段，结合NMR、HPLC、MS、LC-MS 等现代生化分析方法，对完成全基因测序的一个海洋细菌新种BP-2 的海藻酸代谢途径及其关键酶进行研究，明确海藻酸代谢途径中的关键基因及其编码的关键酶，从分子水平深入探讨该菌在海藻酸裂解酶表达，古罗醛糖酸/甘露糖醛酸摄取及其在胞内的代谢过程，阐明 BP-2 降解海藻酸的代谢途径及机理，丰富微生物利用海藻酸机理的理论，为构建不同的海藻酸乙醇发酵工程菌株，为高效转化大型海藻生产生物乙醇提供理论依据。

褐藻胶，又称海藻酸，是最丰富的海洋生物多糖，在生物质能源、医药、食品等领域具有.广阔的前景。海藻酸降解菌可以将海藻酸降解为海藻酸单糖供菌体生长发育需要，然而，海藻酸降解菌的海藻酸代谢途径及其机制还不完全清楚。本项目应用分子生物学研究手段，结合现代生物化学分析方法，对完成全基因测序的一个海洋细菌新种BP-2 的海藻酸代谢途径及其关键酶进行研究，明确了海藻酸代谢途径中的关键基因及其编码的关键酶，从分子水平深入探讨该菌在海藻酸裂解酶表达，褐藻胶其在胞内的代谢过程，阐明 了BP-2 降解海藻酸的代谢途径及机理，丰富微生物利用海藻酸机理的理论，为构建不同的海藻酸乙醇发酵工程菌株，为高效转化大型海藻生产生物乙醇提供理论依据。