基于液滴润湿性异变规律探索血液尿液糖度检测新方法

In recent years, the research of material wetting theory to build intelligent controllable surface to improve the self-cleaning, lyophobic, lubrication, bonding, lyophilic and other properties has become the international frontier and hot spot. However, the current research mainly focuses on the preparation of various functional surfaces and the field of detecting sugar content of blood and urine has not been involved yet, while detecting the sugar content of blood and urine mainly uses chemical techniques in medicine. In order to expand the new application area of wetting theory, a new idea and method based on mutational laws of droplet wetting angle are proposed in this project, and they are used to build for the determination of human blood glucose and urine sugar content detection. In this project, the thresholds of the sugar content of blood and urine of large sample and their thresholds of wetting performance index are researched and determined，the impact laws of different concentrations of sugar in the blood and urine samples on the wetting index are explored and the data models are constructed, the influence rules and mechanism of glucose content in the experimental droplet on the wetting performance are revealed, and the key scientific problems such as the new principle and method of quantitative and qualitative evaluation of blood and urine abnormalities based on the difference of contact angle are proposed. Through the research, the application of the theory of surface wetting is extended to the new field of medical testing and developed into a new stage of precision and convenience. The establishment of the new principle to determine wetting mutation accurately and preparation of bionic surface with hypersensitivity feature have broadened out the new connotation of bionics, which will help to expand our international influence on the application of wetting theory in our country.

近年来表面润湿理论用于构筑智能可控表面以改善自洁、疏液、亲液、润滑、粘结等性能研究已成为国际前沿与热点，其研究主要集中于各种功能表面制备方法，尚未涉及血糖尿糖浓度检测领域，目前对血液与尿液糖度的医学检测主要采用化学技术。为拓展润湿理论应用新领域，本项目提出基于液滴润湿角异常变化规律以构筑用于测定人体血糖与尿糖含量检测的新思路与新方法。研究确定大样本血尿液糖度阈值与其对应的润湿性能指标阈值，探索并构建血液尿液样本中不同糖度对润湿性指标影响规律及数据模型，揭示血液尿液糖度对润湿性能的作用规律与机制，提出基于润湿性指标差异反向推演血尿液糖度异常的定量与定性测判原理与方法等关键科学问题。通过研究把表面润湿理论应用拓展到血液尿液糖度检测新领域，发展到精准便捷检测新阶段。建立与制备润湿异变精准测判方法与具有敏性表征特性的仿生表面，拓宽仿生学新内涵，有助于扩大提升我国在润湿理论应用技术方向的国际影响力。

通过化学刻蚀与低表面能修饰方法制备超疏水铝表面，以旱金莲叶与超疏水铝表面为基底，测量糖尿病患者与正常人血滴与尿滴的接触角与表面张力等参数。试验发现血液尿液样本的表面张力与接触角测量值均随糖度增加而减小，构建了不同糖度液滴接触角异变数学模型，揭示了润湿性指标随液滴糖度的变化机理。试验制备一种能够防止血滴与尿滴污染与破坏基底超疏水性的超滑表面，增加了基底的重复使用次数与使用寿命。根据血滴与尿滴在不同基底上接触角异变规律反求出血液尿液糖度，推演其变化规律，为血糖尿糖浓度检测提供了一种新方法，应用该方法检测的血糖值与血糖仪测量值相比，误差小于5%。因血滴与尿滴的润湿性指标与糖度变化规律为非线性，且需要存储与处理的试验数据量庞大，本研究开发了一个基于血滴与尿滴润湿性异变规律检测血液尿液糖度的数据管理系统，为试验数据存储与转移提供方便，而且能够对试验数据进行分析与修正。