基于不同心肌状态对相关经穴的交感神经反映和受体分子响应规律研究

The correlation between acupints and revelant zang-fu organs is one of the core contents in the acupuncture basis theory. Its holistic view provides the theoretical basis for the fact that when the zang-fu organs are in different state, different reaction of the revelant acupoint may be seen. According to some rsearch reports under the physiological or pathological conditions there are changes of blood flow volume and infrared radiation image, and the revelant receptors and substances of the dermal sympathetic nerve tissues around the acupoints may mediate the acupuncture effect. This project tries to use rats with myocardial ischemia as a research platform, and apply techniques such as Laser Doppler Perfusion Imager detection, infraded radiation image, Real-time PCR, Western blot, laser confocal scanning image, etc. to investigate the differences of the the bio-physical phenemena and the receptor molecular reaction related to sympathenic nerves around the revelant acupoints, and the changes from different bio-physical phenomenon, molecular, gene and protein levels. The aim is to ascertain the differences of the revelant acupoints reflection, and rules alongside time-varying under diferent myocardial conditions, and the critical way and links of the reflection differences, which may expound the scieitific connotation of the correlated acupoint and revelant zang-fu organs under different myocardial conditions, improve the quality of diagnosis and treatment and offer new research perspectives and experimental data.

经穴-脏腑相关是针灸基础理论的核心内容之一。经穴-脏腑彼此相系的整体观，为"脏腑处于不同状态时，相关经穴会出现不同反映"提供了理论依据。有研究发现机体在生理或病理状态下，穴位处血流量和红外辐射成像等有变化；经穴皮肤组织中交感神经相关的受体或物质能介导针刺效应。本课题拟用大鼠心肌缺血损伤作为研究平台，应用血流灌注量、红外辐射成像、Real-time PCR、Western blot、激光共聚焦扫描成像等技术，从生物物理现象、分子、基因和蛋白水平探讨心肌处于正常的生理态、缺血损伤的病理态和电针的干预态下，相关经穴出现与交感神经密切相关的生物物理现象及受体分子响应情况的变化。试图探明心肌在不同状态下，相关经穴出现反映的差异、随时间变化的规律及引起相关经穴出现反映差异的关键途径和环节，为阐释心肌处于不同状态下经穴-脏腑相关的科学内涵，丰富经穴-脏腑相关的临床诊断和治疗，提供新的研究视角和实验数据。

主要研究内容：..从高频或低频电针“内关”穴、高频电针“内关”穴或非经非穴、低频电针“内关”穴或非经非穴等三个方向，分别探讨三个维度（同经异穴、相关和非相关经穴、经穴和非经非穴）在大鼠心肌处于正常生理态、缺血损伤病理态和不同频率电针干预态时穴位反映的特征性变化、分子及受体蛋白和基因水平、表达情况，以及随时间变化的规律。.重要成果：.发现与心脏密切相关的心包经“内关”穴区出现皮肤温度、血流等生物物理反映最明显，其次是“郄门”、“天泉”、“足三里”穴区，“内关”和“郄门”穴区在低频或高频电针经穴1次或3次干预态时，穴区温度和血流显著升高，而“天泉”穴区在低频电针经穴3次后穴区温度和血流显著升高，“足三里”在心肌处于损伤1日或3日时温度显著降低，3日血流显著降低，高频或低频治疗3次后血流显著升高，“阳陵泉”和非经非穴均无显著变化；“内关”穴区NO、CaM和NE等分子响应及α1-AR和β2-AR蛋白/基因含量或表达反应最明显，其次“天泉”，“天泉”穴在治疗3次后穴区β2-AR基因水平显著升高，α1-AR蛋白含量显著下降，而“阳陵泉”和非经非穴均无显著变化。.科学意义.明确心肌在不同状态下，相关经穴出现反映（穴区皮肤温度和血流）的差异，并发现心肌缺血损伤随时间延长及干预次数增加，穴区温度和血流呈现规律性变化，及相关经穴出现分子响应物质反应的差异。为心肌处于不同状态下穴位反映效应及物质基础提供实验证据。