基于蛋氨酸代谢调控的大米蛋白抗氧化机制研究

The precise mechanism by which rice protein exhibits an antioxidant is not fully established. Particularly, the evidence on how rice protein can improve oxidative stress is lacking. To elucidate the antioxidative mechanism exerted by rice protein, the hypothesis that the main modulator responsible for the antioxidative action of rice protein is the metabolic pathway of methionine has been suggested in this study. To test this hypothesis, the present study will be conducted to focus on the regulatory effects of methionine and rice protein on oxidative stress and antioxidative action through in vivo feeding and in vitro liver perfusion. The key questions addressed are: (1) whether can rice protein improve oxidative stress through the metabolic pathway of methionine and (2) how does methionine possess a vital function in improving oxidative stress primarily through the activation of antioxidative mechanism controlled by rice protein? Results will be educed from series investigations, which are dynamic physiological metabolism and gene expression, dietary protein and sole amino acid, whole and molecular level, etc. The aim of this study is to elicit and to reveal the mechanism involved with dynamic oxidative stress and antioxidative action induced by rice protein through methionine metabolism. The significance of this sustainable study is to provide a new theoretical understanding and a novel path to improve the physiological function of rice protein on attenuating oxidative stress, in which the methionine may be the main modulator responsible for the antioxidative mechanism. This study will also lead to a better understanding that the suppression of oxidative stress of rice protein may be a useful target for a new therapy in preventing hyperlipidemia.

本项目针对缺乏应用大米蛋白进行动态抗氧化机制研究的现状，基于Met代谢是调控机体氧化应激、激活内源性抗氧化反应重要途径的特点，结合我们前期系列研究成果，提出"Met代谢是激活大米蛋白内源性抗氧化机能、有效降低机体氧化应激水平的主要作用机制之一"这一假说。拟运用体内整体代谢和体外肝脏灌流的实验模式，采取调控效果与作用机制相结合、动态生理代谢与基因表达调控相结合、天然膳食蛋白与单一氨基酸相结合的研究方法，从整体水平及分子水平上，进行假设理论的探索与论证。旨在通过大米蛋白抗氧化机制及其调控因子的确立，系统、全面地阐明大米蛋白调控氧化应激的作用机制、揭示机体内源性抗氧化机能的激活与代谢调控规律。本项目的科学意义在于通过动态代谢研究方法，进行基于Met代谢调控的大米蛋白抗氧化机制的可持续研究，对开辟应用大米蛋白进行氧化应激干扰、激活机体内源性抗氧化机能的新途径，具有重要理论意义及实际应用价值。

大米蛋白是一种优质的膳食蛋白源。但是，大米蛋白抗氧化机制的研究，迄今为止，国内外尚少见报道。本项目在国家自然科学基金（31371755）的资助下，采取in vivo与in vitro相结合的研究模式，主要以调控效果与作用机制相结合、生理代谢与基因及蛋白表达调控相结合的研究手段，从整体水平及分子水平，重点研究了大米蛋白及蛋氨酸代谢调控机体氧化应激水平、抗氧化作用机制及相关抗氧化调控因子等的作用效果，进而深入解析与阐明了基于蛋氨酸代谢调控的大米蛋白抗氧化机制。.该项目在国内外首次提出了“蛋氨酸代谢是激活大米蛋白调控的内源性抗氧化机能、有效降低机体氧化应激水平的主要作用机制之一”的理论，为今后深入开展功能性大米蛋白的可持续性研究打下了良好的理论基础。该课题的实施为我国大力开发功能性大米蛋白食品奠定了坚实的理论基础，开辟了我国大米精、深、细深加工的广阔途径，具有广阔的应用前景及深远的社会效益。.项目在研期间，已发表学术论著22篇（部），其中，出版专著1部、发表SCI收录论文6篇；申请3项国家发明专利。