基于适配体电化学传感器研究黄体酮检测新方法

Aptamer-electrochemical sensor is a newly developing technique for various promising applications including clinical diagnosis, environmental detection and food security. Importantly, screening and designing the sensitive signal conversion patterns act as the key factor to the Building electrochemical detection method. Aptamer, as target recognition element, possesses the characteristics of high selectivity and cheap. And it holds great potential application in electrochemical detection method. The aim of the project is to design multifunctional aptamer, which could be used as electrochemical sensing probe to establish new method and technology of progesterone detection. Detailedly, the project consisted of the following issues: (i) to construct a new competitive aptamer-electrochemical sensor for the monitoring progesterone based on tyramine signal amplification technique; (ii) to explore multiple signal-amplified aptamer-electrochemical detection strategies by coupling competitive-type displacement reaction mode with substrate catalytic cycle; (iii) to explore a new aptamer-electrochemical sensor based on trigger signal transduction model coupled with visual inspection analysis method. More importantly, this project will provide a useful tool for various scientific research fields relating to national production and public security (e. g. agricultural and animal husbandry production, food safty analysis, clinical diagnosis, etc.).

基于适配体电化学传感探针的检测技术已经在临床诊断、环境检测和食品安全等诸多领域中有着广阔的应用前景。筛选出性能优异并设计灵敏的信号转化模式是构建电化学检测方法的关键所在。适配体作为目标识别元素具有高选择、低廉等特点，展现出应用于电化学检测方法中的巨大潜能。本项目将设计多功能适配体作为电化学传感探针，建立对黄体酮检测的新方法和新技术。具体内容：(1) 建立以酪胺信号放大技术为信号增强策略的新型竞争型适配体电化学传感分析方法；(2) 建立基于竞争置换模式和底物催化循环放大的新型适配体电化学传感分析方法；(3) 建立基于触发型信号转导模式，并联合可视化检测的新型适配体电化学传感分析方法。本研究成果有望在我国食品安全分析和临床检测等国民生产和公共安全密切相关的科学领域发挥重大的作用。

灵敏和快速实现复杂体系中有害物质（如：汞离子、亚硝酸根离子等）和人体生物分子（如：黄体酮、三磷酸腺苷、甲胎蛋白等）检测在临床诊断、环境监测和食品安全等诸多领域有着重要的意义。电化学传感分析方法以其优异的特性成为临床诊断、环境检测和食品安全等诸多领域中的检测利器。信号转导/捕获模式及信号放大对于电化学传感体系的构建至关重要。本项目将新型信号转导/捕获模式偶联信号放大技术引入至检测平台建立一系列传感检测新技术和新方法。具体内容：（i）基于目标触发DNA酶串联体释放的DNA电化学传感体系用于黄体酮的检测研究；（ii）基于酶诱导芬顿反应偶联邻苯二胺氧化的电化学免疫传感体系用于甲胎蛋白的检测研究；（iii）基于便携式血糖仪的均相DNA传感器检测汞离子；（iv）基于核酸酶辅助循环信号放大策略的无标记均相DNA传感体系用于黄体酮的检测研究；（v）基于无标记DNA酶的均相传感体系用于亚硝酸根的快速检测研究。该项研究实现了对水体及加标血清样品中汞离子、亚硝酸根、甲胎蛋白及黄体酮的初步检测应用，为进一步商业化提供理论依据。