ECC儿童唾液中龋易感生物标记物的检测

Early childhood caries (ECC) is the most common oral disease with the highest incidence among chronic child diseases. Sixty-six percent of five-year-old Chinese children have dental decay, and only less than 3% of them are treated. ECC have been shown to severely affect children's oral and general health. Saliva is one of the host factors in the etiology of dental caries. Salivary microbes play an important role in the occurrence and development of caries. Based on the results of previous researches on the etiology of ECC and investigations of relevant salivary microbes, omics approaches of microbes, transcriptomes, proteomes and metabolites were combined to identify biomarkers for caries susceptibility individual in this study. In this study, a longitudinal discovery and independent preclinical validation survey will be conducted to evaluate the performance and translational utilities of salivary microbial, transcriptomic, proteomic and metabolic biomarkers for detection of children with high caries risk.

低龄儿童龋（Early Childhood Caries, ECC）是儿童最常见的口腔疾病，发病率高居儿童慢性病首位。我国5岁儿童患龋率达66%，治疗率不到3%，ECC可对儿童口腔健康及全身健康均造成严重影响。唾液是ECC发生的宿主因素之一，唾液微生物在龋病的发生发展中起重要作用，通过筛选唾液中生物标记物可预测及早期诊断龋病。本项目在既往对ECC病因及相关致龋微生物研究的基础上，将微生物组学技术、转录组学技术、蛋白组学技术及代谢组学技术相结合，对唾液中微生物、差异mRNA、差异表达蛋白和小分子代谢产物进行龋易感性标记物筛选，初步确认ECC易感标志物并测定其含量，纵向追踪个体新发龋数量，计算不同生物标记物对预测ECC的特异度和敏感性，建立较准确的龋风险预测模型，为ECC的预防提供新的防治靶点。