第二信使介导的低温好氧颗粒污泥形成与稳定机制及其调控策略

Aerobic granular sludge under low temperature has become a hot topic in the field of water pollution control. With the aerobic granular sludge under low temperature as the objects, the regulation mechanism of second messenger has been revealed during the formation and stabilization of aerobic granular sludge under low temperature. The regulation strategy mediated by second messenger was proposed. The main contents of this project include: (1) Start-up, operation and mathematical simulation of the aerobic granular sludge system under low temperature; (2) Microbiological interpretation of the low-temperature aerobic granular sludge formation and stabilization mediated by second messenger; (3) Construction of genetically engineered bacteria for second messenger synthesis and hydrolysis and their impact on the aerobic granular sludge system under low temperature; (4) Establishment of the regulation strategy mediated by second messenger for the rapid start-up and stable operation of the aerobic granular sludge system under low temperature. This study belongs to the crossing field of environmental engineering, analytical chemistry and microbiology. The significances of this project are as follows: (1) Constructing a mathematical model of the low-temperature aerobic granular sludge formation and operation, and providing guidance for the rapid start-up and stable operation of the system; (2) Establishing a directed second messenger-mediated strategy for the formation and stabilization of aerobic granular sludge under low temperature based on second messenger regulation mechanism, and providing technical support for the practical engineering application.

低温好氧颗粒污泥技术已成为水污染控制领域的研究热点。本立项以低温好氧颗粒污泥为研究对象，从第二信使调控入手，揭示其对低温好氧颗粒污泥形成与稳定的作用机制，提出第二信使介导的调控方法，内容包括：(1)低温好氧颗粒污泥系统的启动运行及其过程的数学模拟；(2)第二信使介导的低温好氧颗粒污泥系统的启动与运行的微生物学机制解析；(3)第二信使合成与水解基因工程菌的构建及对低温好氧颗粒污泥系统的影响；(4)第二信使介导的低温好氧颗粒污泥系统快速启动与稳定运行策略。. 本研究属于环境工程、分析化学及微生物学的交叉领域，意义在于：(1)建立低温好氧颗粒污泥形成与运行过程的数学模型，为该系统的快速启动及稳定运行提供指导；(2)以第二信使对低温好氧颗粒污泥形成与稳定的机制为依据，建立第二信使介导的低温好氧颗粒污泥定向调控策略，为低温好氧颗粒污泥的实际工程应用提供技术支持。

低温好氧颗粒污泥技术是我国北方低温污水生物处理领域的研究热点之一。本项目以低温好氧颗粒污泥为研究对象，以数学模拟为基础，揭示微生物第二信使对颗粒形成与稳定的生物学机制，建立颗粒快速形成与稳定策略，内容包括：(1)建立了低温好氧颗粒污泥系统的启动运行及其过程的数学模型；(2)结合计算流体力学构建了好氧颗粒污泥系统快速启动与稳定运行策略；(3)阐明了第二信使c-di-GMP对低温好氧颗粒污泥形成与稳定影响的微生物学机制。. 本研究属于环境工程、分析化学、微生物学的交叉领域，意义为：(1)建立了低温好氧颗粒污泥形成与运行过程的数学模型，为该系统的快速启动及稳定运行提供科学指导；(2)以第二信使对低温好氧颗粒污泥形成与稳定的机制为依据，建立了第二信使介导的低温好氧颗粒污泥定向调控策略，为低温好氧颗粒污泥的实际工程应用提供技术支持。