多糖型微生物絮凝剂去除水中重金属离子的机制及其定向合成策略

Microbial flocculant is an advantaged green water treatment agent on heavy metal wastewater treatment. With the study objects of the polysaccharide-based microbial flocculant produced by Agrobacterium tumefaciens F2, the mechanism of the heavy metal removal is revealed. Combined with the analysis of the genes related to its synthesis, the strategy of the polysaccharide-based microbial flocculant oriented synthesis regulation used for heavy metal removal is studied. The main contents of this project include: (1) The mechanism of the heavy metal removal by the polysaccharide-based microbial flocculant based on analytical chemistry and QSAR model;(2) Analysis the gene cluster related to the polysaccharide-based microbial flocculant synthesis by genomics/ transcriptomics technology;(3) Functional determination of flocculant synthesis genes by CRISPR-Cas mediated genome editing technology; (4) Based on the above results, optimization of its synthesis pathway by CRISPR-Cas mediated genome editing technology to achieve its oriented synthesis..This study belongs to the crossing field of analytical chemistry, bioengineering and bioinformatics. The significances of this project are as follows: (1) Revealing the mechanism of the heavy metal removal by the polysaccharide-based microbial flocculant, which is the basis for the oriented synthesis of the polysaccharide-based microbial flocculant; (2) Determination of the gene cluster related to the polysaccharide-based microbial flocculant synthesis, which could fill in gaps in this field; (3) Gene regulation of its synthesis by CRISPR-Cas mediated genome editing technology, which could provide a new idea in the research and development of microbial flocculant.

微生物絮凝剂是一种在重金属废水处理上具有优势的绿色净水剂。本立项以菌株F2产生的多糖型微生物絮凝剂为对象，揭示其去除重金属的机制，结合合成基因的解析，定向合成絮凝剂，内容包括：(1)采用分析化学和QSAR模型的方法探索多糖型微生物絮凝剂去除重金属的机制；(2)采用基因组学/转录组学解析菌株中絮凝剂合成基因簇；(3)利用CRISPR-Cas介导的基因组编辑技术对絮凝剂合成基因进行功能验证；(4)根据上述结果，采用CRISPR-Cas介导的基因组编辑技术优化絮凝剂合成途径，实现定向合成。.本研究属于分析化学、生物工程及生物信息学的交叉领域，意义为：(1)解析多糖型微生物絮凝剂吸附重金属离子机制，为指导微生物絮凝剂的定向合成提供依据；(2)探索多糖型微生物絮凝剂合成基因簇，填补研究空白；(3)采用CRISPR-Cas介导的基因组编辑技术对微生物絮凝剂的合成进行调控，为微生物絮凝剂研发提供新思路。

微生物絮凝剂是一种在重金属废水处理上具有优势的绿色净水剂，本项目以菌株Agrobacterium tumefaciens F2产生的多糖型微生物絮凝剂为对象，揭示其去除重金属的机制，结合合成基因的解析，定向合成絮凝剂，构建絮凝剂合成的调控策略，内容包括：(1)采用现代分析检测方法探索多糖型微生物絮凝剂去除重金属的机制；(2)采用基因组学/转录组学解析菌株中絮凝剂合成基因及途径；(3)利用基因工程技术验证参与絮凝剂合成的基因；(4)根据上述结果，优化絮凝剂合成，构建群体感应调控絮凝剂合成的策略，实现定向合成。. 本研究属于分析化学、生物工程及生物信息学的交叉领域，意义为：(1)解析多糖型微生物絮凝剂吸附重金属离子机制，为指导微生物絮凝剂在重金属污染治理中的应用；(2)探索多糖型微生物絮凝剂合成基因及路径，填补研究空白；(3)采用基因工程技术及群体感应调控策略对微生物絮凝剂的合成进行调控，为微生物絮凝剂研发提供新思路。