基于LIF、bFGF基因探讨鹿茸多肽对周围神经损伤修复的研究

Repair and regeneration of peripheral nerve injury is one of the medical field has not yet been resolved. Therefore, nerve damage defect site exists scarring impede axonal regeneration, slowly growth rate of regenerating axons, that of remote target organs can not effectively dominate and other issues have been focused the research of the peripheral nerve injury. Preliminary studies of our group, PAP initial progress is promoting nerve to repair，it is proposed to apply the LIF/bFGF （Leukemia inhibitory factor to promote axonal regeneration，basic fibroblast growth factor can promote bone marrow mesenchymal stem cell proliferation and promote nerve regeneration and inhibition of scar formation）gene transfected bone marrow mesenchymal stem cells to promote peripheral nerve injury to repair，the use of bone marrow mesenchymal stem cells as vectors to Continue secretion of LIF/bFGF's protein in the role of the injury site, to promote peripheral nerve repair and inhibit scar formation. Application of growth factor drugs - PAP to intervene, gene expression and cell culture techniques, to solve the regenerative capacity of axons in the peripheral nerve injury and repair, scar formation, and nutrients. In order to improve the repair of peripheral nerve injury, thereby restoring the function of damaged tissue and provide a theoretical basis.

周围神经损伤的修复与再生是医学领域至今尚未解决的问题之一。因此，神经损伤缺损部位存在瘢痕形成阻碍轴突再生、再生轴突生长速度缓慢、对远端靶器官不能有效支配等方面问题一直是周围神经损伤的研究热点。本课题组前期研究鹿茸多肽在促进神经修复方面获得初步进展，现拟应用LIF、bFGF基因转染骨髓间充质干细胞，进行促进周围神经损伤修复，利用骨髓间充质干细胞作为载体表达LIF、bFGF蛋白持续作用损伤部位，促进周围神经损伤修复及抑制瘢痕形成，采用生长因子类药物-鹿茸多肽进行干预，以通过基因表达与细胞培养技术，解决周围神经损伤修复过程中神经轴突的再生能力、瘢痕形成，以及营养物质的问题。为提高外周神经损伤修复，从而恢复受损组织功能，提供理论依据。

周围神经损伤的修复与再生是医学领域至今尚未解决的问题之一。神经损伤缺损部位存在瘢痕形成阻碍轴突再生、再生轴突生长速度缓慢、对远端靶器官不能有效支配等方面问题一直是周围神经损伤的研究热点。本课题组前期研究鹿茸多肽在促进神经修复方面获得初步进展，拟应用LIF、bFGF基因转染骨髓间充质干细胞，进行促进周围神经损伤修复，利用骨髓间充质干细胞作为载体表达LIF、bFGF蛋白持续作用损伤部位，促进周围神经损伤修复及抑制瘢痕形成，采用生长因子类药物-鹿茸多肽进行干预，现经过大鼠坐骨神经损伤模型的实验，未观察到鹿茸多肽对外周坐骨神经损伤型大鼠有显著的修复作用，同时，我们对海马神经元进行实验，提取的神经干细胞进行缺氧处理，其存活率显著降低，给予鹿茸多肽处理后，细胞活性水平显著增加。但未观察到转染bFGF和LIF基因后再给予鹿茸多肽治疗其细胞活性有增加的改变。总之，我们的实验未观察到鹿茸多肽修复外周神经损伤的现象，但其可以促进神经生长，修复海马神经元的损伤，这可能是由于鹿茸多肽调节bFGF，LIF基因的表达。