乙肝病毒基因结构及其多样性在宫内传播中的作用

HBV preC/C and preS/S gene were amplified by full-length or long-fragment polymerase chain reaction (PCR) from serum of HBsAg positive pregnant women and their newborns or liver of aborted fetus. These DNA fragments were cloned and sequenced. The results showed all nucleotide sequences of HBV strains contribute to HBV C genome type and adr type. HBV preC/C regions were conservative extremely and the heterogeneity preS/S gene was much higher. HBV nucleotide sequences of neonates and their mothers were identical; the heterogeneity of HBV nucleotide sequences in neonates was lower than that of their mother. The HBV dominant strains and weak strains in mothers can infect fetus and neonates alone or together. The heterogeneity of HBV preS/S gene in HBsAg positive pregnant women whose fetus infected HBV was much higher than that whose neonates didn't infect HBV (p<0.05). This study can offer scientific basis for further study of the relationship between HBV genomic structure and intrauterine transmission.

应用全长PCR扩增技术，研究HBsAg阳性孕妇及其胎儿或新生儿外周血和胎肝HBV基因多样性⒔岷嫌胄刺筒《厩窒ο喙氐腍BV编码区(S、前S1前S2、前C和C区)的结构与变异蛄胁舛ǎ晕捶⑸诟腥镜腍BsAg阳性孕妇为对照，探讨病毒在宫内传播中的作用，寻挠有关的特异性基因标志物，建立筛检方法，为从基因水平阻断传播提供理论与实践基础。