细胞内DNA triplex 介导的转录偶联修复与突变发生的分子机制研究
基本信息
结项摘要
生物细胞基因组DNA不断受到外源和内源性因素的损伤,DNA修复是维持细胞遗传稳定性的重要机制,如果损伤的DNA不能修复将发生突变,最终导致许多遗传病的产生和肿瘤的发生。本研究将建立独特高敏感的可诱导转录的报告基因穿梭质粒系统，通过在不同DNA修复缺陷细胞内形成独特的DNA三联体（triplex）使DNA结构改变或定点损伤，研究由DNA triplex介导的转录偶联修复（Transcription-Coupled Repair,TCR）与突变发生的关系。以期阐明TCR在遗传不稳定性中的作用和分子机制。由于核苷酸切除修复(Nucleotide Excision Repair,NER)在维持细胞遗传稳定性的众多的修复机制最为重要,而TCR是NER中修复转录基因损伤的重要途径，因此研究triplex介导TCR与突变发生的分子机制，有助于深入理解许多疾病尤其是肿瘤发生机制，具有重要的理论意义。
项目摘要
项目成果
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数据更新时间：2023-05-31
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