基于有机阴离子转运肽表达的苍附导痰汤防治痰湿型pcos机制的研究

Polycystic ovary syndrome (PCOS) is one of the most intractable endocrine and metabolic disorders in women of reproductive age. The mechanism of PCOS has not yet been clarified. It has linked to multiple systems, such as genetic, endocrine and metabolic. Clinical research find that dampness is closely related to the occurrence and development of obese PCOS and dispelling dampness is an effective method for prevention of obese PCOS. However, its biological basis is unclear. In our previous study, we found that the expression of organic anion transfer peptide(oatp) in body organization was closely related to the transportation and transformation of dampness, furthermore , our preliminary experiment proved the expression of oatp4a1 was abnormal in endometrial and ovarian tissue of rat model induced obese PCOS . From what has been discussed above ,the treatment of research is Cangfudao tan decoction derived from Ye Tianshi's women diseases , which therapeutic principle is “samba open bitter drop and dispelling phlegm ” ,we will copy the phlegm PCOS rat model , use RT-PCR、Western-blot and IHC technologies to detect the expression of oatp in phlegm PCOS model rats’organizations and observe the relationship between the abnormal expression of oatp and the occurrence and development of obese pcos before and after treatment of Cangfudao tang decoction. Our objective of study is to explore new treatment strategy of Cangfudao tan decoction for phlegm PCOS . To reveal "obese people much phlegm, treatment for phlegm wet" scientific connotation, furthermore ,in order to provide a basis of traditional Chinese medicine in the treatment of PCOS.

多囊卵巢综合征（pcos）是育龄期妇女难治性疾病，其发病机制尚未阐明，目前研究主要集中在遗传、内分泌、代谢等系统上，但临床研究发现痰湿内蕴与PCOS关系密切,且“从痰论治”疗效确切，却少有从痰湿途径探讨pcos发生发展的分子机理。在前期研究中发现脾主运化本质的相关指标有机阴离子转运肽（oatp）与湿浊转运密切相关，并在预实验中观察到pcos大鼠子宫内膜及卵巢组织oatp4a1表达异常。本课题即以《叶氏女科》中以“辛开苦降、祛湿豁痰”立法的效方苍附导痰汤为干预手段，以痰湿型PCOS大鼠为研究对象，应用蛋白及基因检测技术，寻找模型大鼠相关组织oatp表达的规律，同时比较分析干预后组织中oatp表达的变化，探讨痰湿内环境湿浊转运障碍对PCOS的影响，旨在从痰湿转运角度探讨苍附导痰汤防治PCOS的分子生物学机制，揭示 PCOS “肥人多痰、从痰论治”的科学内涵，为中医药治疗PCOS提供依据。

多囊卵巢综合征（pcos）是育龄期妇女难治性疾病，其发病机制尚未阐明，目前研究主要集中在遗传、内分泌、代谢等系统上，但临床研究发现痰湿内蕴与PCOS关系密切,且“从痰论治”疗效确切，却少有从痰湿途径探讨pcos发生发展的分子机理。在前期研究中发现脾主运化本质的相关指标有机阴离子转运肽（oatp）与湿浊转运密切相关，并在实验中观察到pcos大鼠子宫内膜及卵巢组织oatp4a1、oatp2b1、oatp3a1表达异常。本课题即以《叶氏女科》中以“辛开苦降、祛湿豁痰”立法的效方苍附导痰汤为干预手段，以痰湿型PCOS大鼠为研究对象，应用蛋白及基因检测技术，寻找模型大鼠相关组织oatp表达的规律，同时比较分析干预后组织中oatp表达的变化。结果显示：与空白组比较，模型组肝、脾、肾组织中oatp3a1、oatp2b1表达量明显下降(P﹤0.01)，肺组织中oatp3a1无明显变化(P>0.05)；与模型组比较，CFDTT高剂量组和二甲双胍组大鼠肝、脾、肾组织中oatp3a1、oatp2b1明显升高(P﹤0.01)，CFDTT低剂量组肝、脾、肾组织中oatp3a1、oatp2b1表达轻度升高(P﹤0.05)，CFDTT高剂量组与二甲双胍组比较差异无显著性(P>0.05)。研究表明痰湿内环境湿浊转运障碍对PCOS的影响，从痰湿转运角度探讨苍附导痰汤防治PCOS的分子生物学机制，揭示 PCOS “肥人多痰、从痰论治”的科学内涵，为中医药治疗PCOS提供依据。