蛋白印迹液晶光致变色膜的构筑及印迹过程可视监测方法建立

The protein imprinting and monitoring is always a long-term challenge and scientific problem in the field of molecular imprinting. It has promoted the pace of application of protein imprinting material to the practical application whenafter surface protein imprinting arised. Although, we still have little knowledge of imprinting and removing macromolecules such as protein. Basically, the lack of intuitive and effective means of monitoring is the main cause. Applicants have recently found that the weak interaction between liquid crystalline photochromic materials and proteins can affect the optical properties of materials. This finding provides a visual and quantifiable method for the development of protein imprinting materials. But there are several urgent problems to be resolved such as the synthesis of liquid crystal materials met the sensing requirements, effective combination of protein imprinting and sensing materials and how to establish a monitoring method through the corresponding scientific issues. Therefore, the project proposed to carry out research around the following: Design of liquid crystal polymer and synthesis of photochromic film to explore effective methods of membrane protein imprinting; Evaluation of surface imprinted liquid crystal photochromic film to study the corresponding relationship among adsorption amount, membrane color and spectral data; Monitoring method establishment of protein imprinting process to explain the mechanism of protein imprinting and recognition. The completion of this project will enrich the types of protein imprinting materials to provide a novel visual and quantifiable method for the printing process monitoring, to improve the mechanism of protein imprinted recognition, and to provide basic theoretical guidance for the promotion and application of protein imprinting materials or technical supports.

蛋白印迹过程追踪与监测始终是分子印迹领域长期面临的挑战和科学难题。表面蛋白印迹推进了印迹材料向实际应用的步伐，但迄今人们对蛋白进出印迹位点的过程知之甚少，究其原因是缺乏直观有效的监测手段。申请人近年研究发现液晶光致变色材料与蛋白间的弱相互作用可影响材料的光学特性，该发现为可视、可量化蛋白印迹监测材料的开发提供了思路。但存在满足传感要求液晶材料的合成、印迹与传感材料的有效复合、如何建立监测方法等科学问题亟待解决。因此，本项目提出开展液晶分子与光致变色膜的设计制备研究，探究膜表面有效蛋白印迹方法，综合评价表面蛋白印迹液晶光致变色膜的性能，深入研究吸附量与膜颜色及光谱数据间的对应关系，建立印迹过程监测方法，探究蛋白印迹识别机制。本项目的完成，将丰富蛋白印迹材料种类，为印迹过程监测提供一种新颖的可视、可量化的方法，完善、明确蛋白印迹识别机理，更为蛋白印迹材料的推广应用提供基础性理论指导和技术支撑。

蛋白印迹过程追踪与监测始终是分子印迹领域长期面临的挑战和科学难题，因此，本项目针对蛋白印迹识别过程监测和识别机理探究、新型高效表面蛋白印迹材料开发与评价开展了深入的研究。主要研究内容包括：（1）设计并合成了具有光致变色性能的液晶分子单体，制备了蛋白印迹液晶光致变色高分子膜，评价了其对印迹过程的示踪性能；（2）通过具有可逆性二硫键偶联模板蛋白技术，首次开发了通过动态键结合荧光证明印迹位点存在的方法，揭示了特异性识别作用的发生；（3）首次开发了具有自驱动性能的表面印迹管状碳纳米纤维，高的比表面积、合理的孔道结构、自驱动的性质在提高材料吸附量的同时加速了吸附进程；（4）构筑了一种以磁性管状纳米纤维为载体的BSA表面印迹材料，其兼具自驱动吸附性能和磁响应性，提高吸附量、加速吸附进程的同时简化了分离过程；（5）通过超声雾化技术制备了褶皱形貌磁性石墨烯载体，合成了新型BSA表面印迹磁性石墨烯微球，该印迹微球展现了高的选择性；（6）制备了具有双重效应锚固BSA模板的磁性蛋白印迹微球，阐明了基于金属配位和多重官能团配体的双重效应对印迹识别效果的影响规律；（7）结合高內相乳液与DPE控制聚合技术，合成了一种新型的温敏型表面蛋白印迹聚合物整体柱，该材料具有选择性和吸附量高、操作简单等优势。通过三年的研究，取得如下主要成果：（1）申请国家发明专利11项，已获授权2项；（2）发表标注资助的SCI收录论文38篇；（3）培养博士生和博士后各1人，硕士生2人。