基于打印技术调控SERS热点的阵列型试纸及其对农药残留的快速分析

Pre-finished Youth Foundation has made significant progress in the rapid enrichment and removal of pesticide residues. The project will focus on the difficulties of component complex, tedious process and high cost in the identification and rapid detection of pesticide residues in actual samples. The proposed development of technology based on surface-enhanced Raman scattering (SERS) Raman sensitive probe and print regulation of Raman array type test strips to achieve the synchronization of a multi-component pesticide rapid detection of: (1) the design and preparation of the metallic nanostructures having hot spots enhancement, through the regulation of the gap and the component, to achieve the maximization of the Raman enhancement effect, to clarify the relationship between hotspot structure and SRES enhanced effect; (2) exploration of the nanoprobes functional pathway, through the modification of the surface ligands and artificial antibodies, to achieve the selective and ultra-sensitive Raman signal output of combined trace pesticides; (3) preparation of the Raman sensitive probe ink suitable for inkjet printing, regulation of the ink ingredients, concentration and the number of printing layers, printing the multi-component pesticide sensitive array type strips; (4) establishment of Raman fingerprint identification modes of pesticide residues using the SERS array type test strips, as well as multi-component fast Raman spectroscopy identification and quantitative analysis method. The expected results of the project will provide a scientific basis and technical approach of the rapid detection of trace harmful substances in environment and food.

前期已结题的青年基金在农残的快速富集与去除等方面取得显著进展。本项目将重点针对实际样品中农残鉴定及快速检测中的组分复杂、过程繁琐和成本高等难题，拟发展基于表面增强拉曼散射（SERS）技术的拉曼敏感探针及打印调控的拉曼阵列型试纸实现对农残的快速高通量检测：（1）设计和制备具有热点增强的金属纳米结构，通过对间隙和组分的调控，实现拉曼增强效应最大化，阐明热点结构和增强效应的相互关系；（2）探索热点纳米探针的功能化途径，通过表面配体及人工抗体的修饰，实现对痕量农残的快速选择性结合和超敏感的拉曼信号输出；（3）制备适合喷墨打印的拉曼敏感探针墨水，通过对墨水成分、浓度和打印层数的调控，印刷对多组分农残敏感的阵列型试纸。（4）建立SERS阵列型试纸的农残拉曼指纹识别模式，以及多组分农残的快速拉曼光谱鉴定和定量分析方法。本项目预期成果将为环境和食品中痕量有害物质的快速检测提供科学依据和技术途径。

本项目以农药残留和重金属离子的痕量检测为目标，发展了基于表面增强拉曼和荧光检测试纸等检测技术，实现了对多种农药残留以及重金属离子的快速超痕量以及可视化检测。主要发现点和成果如下：（1）制备了具有拉曼热点可控的拉曼检测试纸平台，实现了对农药福美双、乙基对硫磷和爆炸物TNT的超痕量检测；（2）构建了一种反应性拉曼探针，为细胞内自由基的成像及药物代谢的监测提供了新的手段；（3）发展了不同类型的比率荧光检测试纸，实现了对环境中有毒有害物质H2S、Hg2+、Cu2+、以及F-等的可视化超敏感检测。上述研究工作取得了多项具有创新性和系统性的研究成果，发表论文19篇，申请专利2项。基于本项目及研究室其他研究项目的研究成果，本研究室目前与深圳市法兰智联股份有限公司成立了“环境快速检测联合研究中心”，获得每年80万元的研究经费支持。