外泌体（Exosome）在小肠上皮损伤修复的作用机制及甘草的干预研究

Small intestine is the organ playing different roles, including digestive and absorptive functions, maintenance of a barrier against noxious antigens and bacteria, and secretion of water and electrolytes. It has been identified to be the "motor" of multiple organ dysfunction syndrome (MODS) and some critical condition. Many pathological stresses result in small intestine injury. To maintain its Homeostasis, repair must be initiated immediately after stress. Intestinal repair are contributed mainly by migration of epithelial cells and proliferation and differentiation of intestine stem cells, Cell communication, and Wnt, Notch, miRNA-mediated signaling pathway is involved in the process. Exosome carrying lipids, proteins and RNA play a central role in communication between cells, and can activate Wnt, Notch, miRNA-mediated signaling pathway. The current study focus on exosome in intestinal injury and repair after stress. We will analyze components of exosome secreted by stressed intestine epithelial cells ,and observe their effect on proliferation and differentiation of stem cell and migration of intestinal epithelial cells, and observe activation and transduction of Wnt, Notch , miRNA-mediated signaling pathways. observe the while observing the effect of the active ingredient in licorice and this is. Clarify Exosome in intestinal epithelial injury and repair stress the role and mechanism analysis of active ingredients stress prevention and treatment of intestinal epithelial targets, while filtering out licorice, to clarify its mechanism of action. Therefore we can clarify the role of exosome in intestinal injury and repair after stress, and find oud the potential targets of prevention and therapy of injury and repair. At the same time, we will study the effects of licorice and its active constituents on this pathway, discover their mechanisms of protecting and repairing effects on intestine stress.

小肠是消化吸收、分泌和免疫防疫的重要器官，是多种疾病尤其是MODS的发生和转归中心。多种因素能导致小肠上皮应激损伤，需迅速启动上皮细胞移行覆盖和小肠干细胞增殖分化进行修复，才能维持上皮完整，细胞通信及Wnt、Notch、miRNA介导的信号通路在此有关键作用。外泌体（Exosome）可携带脂质、蛋白、RNA在细胞间进行通信，并能激活Wnt、Notch信号通路。项目在原有基础上探讨Exosme在小肠上皮应激后损伤修复的作用，分离应激损伤的不同上皮细胞分泌的Exosome，分析其成分，作用于小肠干细胞和上皮细胞，研究Wnt、Notch、miRNA介导的信号通路的激活和转导，观察小肠干细胞的增殖分化和上皮细胞的迁移，同时观察甘草及其有效成分在此的作用。阐明Exosome在小肠上皮应激损伤修复的作用和机制，分析预防和治疗小肠上皮应激的靶点，同时筛选出甘草的有效成分，阐明其作用机制。