模拟微重力环境对牙龈卟啉单胞菌生物学效应影响的研究

In recent years, the rapid development of manned space flight, in the face of the manned space engineering central control and life support is receiving more and more attention. Long-term space flight has put forward higher requirements for space environment for human survival, while the astronauts normal health guarantee and in the space environment for different physiological and psychological stress make rapid and effective response, also become the main goal and focus in recent years in space biology and medical research. Periodontal health is more important for astronauts. The astronauts to withstand harsh conditions, the changing gravitational environment complex space environment has put forward higher requirements for the body's ability to adapt, and increased the number of diseases including periodontal disease incidence. Bacterial factor is the initiation factor of the onset of periodontitis, Porphyromonas gingivalis ( P .Gingivalis ) is the important sign of periodontitis bacteria, increasing their number indicates that the recurrence of adult periodontitis. In the space environment, the occurrence and development of periodontitis by microgravity affect whether astronauts Porphyromonas gingivalis virulence, pathogenicity and thus affect the proliferation and differentiation of cells, eventually led to changes in occurrence and development of periodontitis, needs to be further explored, with expectations for the future to provide effective space and provide a theoretical basis for rational countermeasures

近年载人航天事业迅速发展，面对后续载人航天工程中环控生保问题也受到越来越多的关注。长期太空飞行对于空间人类生存环境提出了更高的要求，而宇航员正常身体健康状况的保证及在空间环境中对于不同生理心理应激作出快速而有效的应对，也成为近年来空间生物学及空间医学研究的主要目标及重点。牙周健康对于宇航员更为重要。宇航员要承受各种极其苛刻的条件，复杂的太空环境中不断变换的重力环境对于机体的适应能力提出了更高的要求，并且增加了很多疾病包括牙周疾病的发病率。细菌因素是牙周炎发病的始动因子，牙龈卟啉单胞菌( P .gingivalis )是牙周炎重要的标志细菌，其数量的增加预示着成人牙周炎的复发。在空间环境中，宇航员牙周炎的发生发展是否通过微重力影响牙龈卟啉单胞菌的毒力、致病性从而影响到细胞的增殖分化，最终导致牙周炎发生发展的变化，需要进一步探讨,以期望为未来提供有效的空间对抗措施提供有理的理论依据。

近年载人航天事业迅速发展，航天员正常身体健康状况的保证及在空间环境中对不同生理心理应激作出快速而有效的应对，使人们对空间生物学及空间医学研究的关注越来越多。细菌因素是龋病、牙周疾病等疾病发病的重要因素，变异链球菌是重要的致龋菌，牙龈卟啉单胞菌是牙周疾病重要的标志细菌。本研究针对微重力环境可能导致细菌的生物学效应的变化，采用旋转细胞培养系统建立模拟微重力细菌培养模型，通过吸光光度法观察细菌的生长情况，通过扫描电镜、透射电镜观察模拟微重力对细菌的形态学的影响。按照计划，完成实验内容，结果观察到模拟微重力环境可加快细菌的生长，模拟微重力环境下细菌的形态学发生明显变化，提示模拟微重力环境对细菌的生物学效应有一定的影响。