马铃薯块茎-软腐病菌互作过程中线粒体内膜两条能量散失途径的运行、调控机制及其病理学功能研究

One of the most important characteristics of the difference of respiratory system between higher plants and higher animal is the plant mitochondrial membrane where has a special energy dissipation way - plant cyanide-resistant respiration alternative pathway, more and more studies have found that the energy dissipation pathways play an important role in plant disease resistance responses. In recent years, researchers found another energy dissipation pathway in plant mitochondrial metabolic - plant uncoupling mitochondrial protein pathway, where the plant uncoupling mitochondrial proteins (PUMPs) as the active site and cause mitochondrial proton leak, so as the second energy dissipation pathways involved in plant metabolism, but it's pathological function is unknown. However, similar uncoupling mitochondrial proteins were shown to have an important role in the pathophysiology in animal body. The puzzle is ：why plant mitochondria exist two energy dissipation ways functional similar at the same time? This project is based on previous work in this laboratory, through the process of potato mitochondrial membrane two energy dissipation way system research interaction between potato tube and soft rot bacteria, to obtain a thorough understanding of the complex function of plant mitochondrial membrane and to enrich the plant pathogen interaction theory.

高等植物呼吸系统区别于高等动物呼吸系统的最重要特征之一是植物线粒体内膜上存在一条特殊的能量散失途径-植物抗氰交替呼吸途径，越来越多的研究发现这条能量散失途径在植物抗病反应中发挥了重要作用。近年来，研究者又在植物线粒体内膜上发现了另外一条能量散失代谢途径-植物解偶联线粒体蛋白途径，其以植物线粒体解偶联蛋白（PUMPs）为活性位点且能够引起线粒体质子漏，从而作为第二条能量散失途径参与植物代谢，但病理学功能不详。然而，类似的解偶联线粒体蛋白在动物体内被证明具有重要的病理学意义。令人困惑的是：植物线粒体内为什么同时存在功能相似的两条能量散失途径？本项目拟在本实验室前期工作基础上，通过对马铃薯块茎-软腐病菌互作过程中马铃薯线粒体内膜上两条能量散失途径的系统研究，深入了解植物-病原菌互作过程中两条能量散失途径运行的调控机理及独特的病理学功能，进一步丰富植物呼吸代谢参与植物-病原菌互作的理论基础。