Notch1信号通路调控牙周韧带干细胞向雪旺细胞分化的作用和机制研究

The reason of low mastication sensitivity possibly owes to lack of sensors and sensory nerve endings around dental implants. Schwann cells, with the potential to conduct the regeneration of nerve endings and sensors, may have the ability to improve the mastication sensitivity. However, it’s harvesting and purification is difficult, the application of SCs is limited. As it is known in recent years, PDLSCs have the potential of differentiation, so may be applied on theregeneration of peri-implant never tissue. Our former researches have shown the differentiation possibility from PDLSCs to SCs, but its mechanism is still unknown. Because Notch signal pathway has a important role in nerve regeneration and stem cells differentiation, so we want to investigate whether Notch1 signal pathway participate in and regulate the differentiation from PDLSCs to SCs. In a word, we hope stably differentiated PDLSCs can replace SCs to repair the impaired peri-implant nerve endings, so we can improve the osseoperception and mastication sensitivity of dental implants to mimic nature teeth, meanwhile, we also hope our results could inspire further mechanism investigation about theoretically prepare for regenerating peri-implant nerve tissue in the future.

牙种植体周神经纤维末梢和感受器少是种植体咀嚼敏感性低导致种植体损伤的重要原因。雪旺细胞(SCs)可引导周围神经末梢和感受器再生，提高种植体咀嚼敏感性，但其来源和纯化极其困难。近年来牙周韧带干细胞(PDLSCs)多向分化潜能备受关注，有望替代SCs应用于种植体周神经组织工程。我们前期通过化学因子成功诱导PDLSCs向SCs分化，但调控其分化的机制不清。Notch1信号通路在神经发育和干细胞神经向分化中具有重要作用，近年来发现其在PDLSCs向SCs分化中也同样发挥重要作用。本研究拟在前期研究基础上优化分化条件，利用细胞免疫荧光等方法研究Notch1信号通路关键蛋白表达情况揭示其调控机制，以期实现PDLSCs雪旺细胞向的稳定分化，替代SCs修复种植体周神经缺损，提高牙种植体的骨感知能力和咀嚼敏感性，实现模拟天然牙的感觉功能，为重建牙种植体周神经组织工程提供理论基础。

牙种植体周神经纤维末梢和感受器少是种植体咀嚼敏感性低导致种植体损伤的重要原因。雪旺细胞(SCs)可引导周围神经末梢和感受器再生，提高种植体咀嚼敏感性，但其来源和纯化极其困难。近年来牙周韧带干细胞(PDLSCs)多向分化潜能备受关注，有望替代 SCs 应用于种植体周神经组织工程。我们前期通过化学因子成功诱导PDLSCs 向 SCs 分化，但调控其分化的机制不清。Notch1 信号通路在神经发育和干细胞神经向分化中具有重要作用，近年来发现其在 PDLSCs 向 SCs 分化中也同样.发挥重要作用。本研究在前期研究基础上筛选出 PDLSCs 向 SCs 优化最佳分化条件，利用细胞免疫荧光等方法研究 Notch 信号参与 PDLSCs 向SCs 分化过程的调控，上调或抑制该通路影响 PDLSCs 神经向的分化过程，以期实现 PDLSCs 雪旺细胞向的稳定分化，替代 SCs 修复种植体周神经缺损，提高牙种植体的骨感知能力和咀嚼敏感性，实现模拟天然牙的感觉功能，为重建牙种植体周神经组织工程提供理论基础.