多聚微囊化转脑啡肽基因细胞的镇痛效应和免疫耐受研究

The project induclinding three parbs: 1. The making of modified cells of proenkephalin gene and dealling with microencapsulative cells. 2. Alive condition of microencapsulative cells、tumorigenic、 secrete function. 3. The reach of microencapsulative cells are used in chronic pain we carried on the plan in the main.With the new developping of microencapsulation technology. We used Alginate as the materical in microencapsulation we abandoned polyacrylonitrile and polyvinyl chloride (PAN-PVC) which was planned to be used. Detas show that Alginate microencapsulation is easy to be made, the size is easy to be controlled. And it's physics and chemistry quality are stable, The Alginate microencapsulation is suit for cell transplantion.

细胞镇痛是慢性疼痛治疗的一种新方法。本课题将采用正常细胞系体外转染人前脑啡肽原基因后微囊化处理，研究微囊小体对细胞的存活、增殖、分泌功能和致瘤性的影响及对细胞的免疫保护作用；将微囊化细胞植入慢性疼痛模型动物的蛛网膜下腔，观察该细胞镇痛的有效性和安全性，探讨解决细胞镇痛技术的细胞来源困难和存在免疫排斥这两大难题的方法。