糖肽疫苗的设计、合成与性质研究

Vaccine is the most promising method for the prevention and cure of diseases, such as cancer and bacterial or viral diseases. Choosing the right antigen is the primary factor for the success of vaccine. With the ever-increasing level in our understanding of basic immune mechanisms, glycoprotein is considered as the most important antigen in vaccine development. As its heterogeneity, glycoprotein obtained by bioseparation technology is not advisable for vaccine development. Chemical synthesis is powerful to produce homogeneous glycopeptide with selective glycosylation modification. Moreover, chemical synthesis is feasible to combine glycopeptide antigens and immune adjuvant for vaccine design. One of the major problems in developing an efficient vaccine is the weakness of specific immune responses. Various vaccine design strategy have been proposed to overcome this weak immune response, which included multiple-component vaccines, multiple-valent vaccines are proposed in this proposal.

治疗性疫苗在疾病治疗、免疫机制研究等方面具有重要作用，糖肽肿瘤疫苗在肿瘤治疗、诊断以及相关免疫研究中的应用已愈来愈受到关注。目前糖肽类疫苗存在免疫原性低、疫苗结构复杂、免疫机制不清楚等不足。. . 本项目拟从抗原相关蛋白（如粘蛋白MUC1、MUC4等）的结构入手，分析目标蛋白immunodominant motif，设计、合成（化学法和酶法）并筛选出具有很好免疫活性的抗原结构，利用多组分疫苗、多价疫苗、聚合物嵌段疫苗等思路构筑新型、高效的疫苗体系，并利用这些糖肽疫苗分子作为探针，探究免疫过程的一些机制。该研究将为获得高免疫活性的糖肽疫苗，并探讨糖肽疫苗免疫机制打下很好的基础。

治疗性疫苗在疾病治疗、免疫机制研究等方面具有重要作用，糖肽肿瘤疫苗在肿瘤治疗、诊断以及相关免疫研究中的应用已愈来愈受到关注。目前糖肽类疫苗存在免疫原性低、疫苗结构复杂、免疫机制不清楚等不足。.本项目从肿瘤相关糖抗原相关蛋白MUC1的结构入手，分析目标蛋白immunodominant motif，设计、合成（化学法和酶法）具有很好免疫活性的抗原结构。为有效地活化抗原呈递细胞，增强免疫反应，我们构筑了基于MUC1糖肽的多组分疫苗、多价疫苗、聚合物嵌段疫苗等新型、高效的疫苗体系，以发展高效的治疗性肿瘤疫苗。并利用这些糖肽疫苗分子作为探针，探究免疫过程的一些机制。该研究将获得高免疫活性的糖肽疫苗，并探讨糖肽疫苗免疫机制打下很好的基础。