基于化学发光新体系的毛细管电泳分离检测酚类化合物卫生分析新方法研究

The pollution problem of phenolic compounds is becoming more and more serious with the development of the industry. Considering that the phenolic compounds in environmental and biological samples are usually in low concentration with multiple coexistence, the establishment of accurate and reliable detection methods is therefore the key technology for evaluating the ecological influences of phenolic compounds. Capillary electrophoresis is better than HPLC and GC in separating structure similar phenolic compounds, but high sensitive detection method is always needed. Chemiluminescence method has the unique potential. In our previous study, we firstly reported a novel chemiluminescence system, i.e.,the Ag(III) complex-luminol system, which has the advantages of high emission quantum yield and very fast reaction rate. Recently, we found that the analyte which has phenolic structure can dramatically enhance or quench the chemiluminescent reaction between luminol and the Ag(III) complex, even if it is only present in trace level. Based on this finding,the presented research is to build high sensitive and fast capillary electrophoresis-chemiluminescence methods for phenolic compounds analysis. In addition, based on our previous work, we are going to research the dose-effect relationship of some phenolic compounds in causing abnormal neurotransmitter secretion or oxidase activity change, and therefore to provide scientific basis for the research on biological effects of phenolic compounds.

酚类化合物的污染问题随着工业的发展日益突出，由于其在环境和生物样品中存在浓度低且共存干扰物多，因此建立准确可靠的检测方法成为正确评价酚类化合物对环境生态影响的关键技术。毛细管电泳法在分离结构相似的酚类物质方面较HPLC和GC具有优势，但需要高灵敏度的检测方法与之匹配，其中化学发光法独具潜力。在前期工作中，我们首次提出了Ag(Ⅲ)配合物-鲁米诺化学发光新体系，其具有发光效率高等优点。研究中发现：具有酚类物质结构特征的目标分析物痕量存在时即对该化学发光新体系产生显著增强或淬灭发光的作用。基于此，本研究拟将高灵敏度的化学发光检测手段与高分辨率的毛细管电泳技术联用，建立灵敏、快速的毛细管电泳-化学发光酚类物质检测新方法。并在前期研究工作的基础上，探索某些酚类物质引起神经递质浓度和氧化酶活性改变的“剂量-效应”关系，为研究某些酚类化合物的生物学效应提供科学依据。

酚类化合物的污染问题随着工业的发展日益突出，由于其在环境和生物样品中存在浓度低且共存干扰物多，因此建立准确可靠的检测方法成为正确评价酚类化合物对环境生态影响的关键技术。毛细管电泳法在分离结构相似的酚类物质方面较HPLC和GC具有优势，但需要高灵敏度的检 测方法与之匹配，其中化学发光法独具潜力。在前期工作中，我们首次提出了Ag(Ⅲ)配合物鲁米诺化学发光新体系，其具有发光效率高等优点。研究中发现：具有酚类物质结构特征的目标分析物痕量存在时即对该化学发光新体系产生显著增强或淬灭发光的作用。基于此，本研究将高灵敏度的化学发光检测手段与高分辨率的毛细管电泳技术联用，建立了灵敏、快速的毛细管电泳-化学发光检测萘酚同分异构体、己烯雌酚、双烯雌酚等酚类物质的新方法，将其应用于实际生物样品与环境样品检测，结果令人满意。此外，在前期研究工作的基础上，还探索了双酚A引起神经递质浓度和氧化酶活性改变的“剂量-效应”关系，为研究其生物学效应提供了科学依据。