三叉神经节低阈值机械感受神经元对牙移动疼痛的调控机制

Malocclusion is sever disease with characteristics of abnormality in tooth alignment, craniofacial shaping, tooth and occlusion function and compared high incidence rate. Orthodontic treatment is of great significance in public health improvement. However, tooth movement pain hindered the popularity of orthodontic treatment. A effective orthodontic pain control method has been essential for orthodontic treatment. Low threshold mechanoreceptors, LTMRs are responsible of taking innoxious stumili and turned them into tactile in normal condition and pain in the pathological condition. We proposed that LTMRs participated the tooth movement pain regulation via IL-1βand TNFαmediated peripheral hypersensitivity and KCC2 and NKCC2 dependent central hyperplasia. This project takes LMTRs as the research object, by using in vivo neuronal activity recording technique combined with single discharge cell electrophysiology, molecular biology techniques, to investigate the mechanism of LMTRs involved in pain modulation of tooth movement and seek new targets for pain control on this basis, provides research basis and reliable theoretical basis for the optimization of orthodontic pain control scheme, open up new ways of orthodontic pain control.

牙颌畸形是牙齿排列、颅面形态、牙颌功能异常的严重疾病，发病率高。正畸治疗对提高人口健康水平具有重要意义，而矫治力引起的牙移动疼痛严重影响了正畸治疗的普及，有效地控制疼痛已成为正畸治疗的关键。低阈值机械感受神经元（Low threshold mechanoreceptor, LTMRs），负责接收非伤害性机械刺激，在正常生理情况下形成触觉与压力觉，在病理情况下形成痛觉。目前尚未见LMTRs对牙移动疼痛调控的相关的报道。因此我们提出假设，LMTRs通过IL-1β、TNFα介导的外周敏化途径及KCC2及NKCC1依赖的中枢敏化途径参与牙移动疼痛调节。本项目以LMTRs为研究对象，采用在体神经元单体放电活动记录技术结合细胞电生理学、分子生物学技术，探讨LMTRs参与牙移动疼痛调控的机制并在此基础上寻求控制疼痛的新靶标，为优化正畸疼痛控制方案提供可靠的研究基础和理论依据，开辟正畸疼痛控制的新途径。