冠心病患者快速进展新发病变的分子机制研究

Percutaneous coronary artery interventional treatment can significantly improve the patients' prognosis of acute coronary syndrome, there is still some patients will occur again in the adverse cardiovascular events, and recurrent myocardial ischemia events can caused by recurrence of the criminal lesions, may also be caused by the rapid progress of the new lesions. Lead to rapid progression of new lesions of recurrent adverse cardiovascular events of great harm, but less of the mechanism of reports. The known transcription factor TCF21 and the new type of metalloproteinase ADAMTS7 gene may be related to the rapid progression of new lesions. The project intends to from clinical screening and new lesions the polymorphic site positive polymorphisms of related gene tcf21 and ADAMTS7, gene cloning, expression in vitro and to study the influence of tcf21 and ADAMTS7 function of encoded protein amino acid polymorphism loci, which lead to the change of proliferation and migration of vascular smooth muscle cells (VSMCs), and ultimately to clinical verification of node. The project for acute coronary syndrome after PCI levy the prevention and treatment of recurrence of adverse cardiovascular events provide a kind of new idea, new target point, to the maximum extent to reduce due to individual differences and lead to recurrence of adverse cardiovascular events to provide basis of theory and research.

急性冠脉综合征经皮冠状动脉介入治疗能显著提高患者的预后，但是仍有部分患者再次发生不良心血管事件，而不良心血管事件的复发可以由罪犯病变的复发所导致，也可能是因为快速进展的新发病变所引起。快速进展新发病变导致再发不良心血管事件的危害极大，但是对其机制性的探讨报道较少。已知转录因子TCF21和新型金属蛋白酶ADAMTS7基因可能与新发病变的快速进展相关。本项目拟从临床水平筛选出与快速进展新发病变相关基因TCF21与ADAMTS7的阳性多态性位点，进行基因克隆，体外表达，探讨多态性位点上的氨基酸改变对TCF21与ADAMTS7基因编码蛋白结构和功能的影响，从而改变血管平滑肌细胞(VSMCs)的增殖和迁移，最终以临床验证为研究结点。本项目将为急性冠脉综合征患者PCI术后再发不良心血管事件的防治提供一种新思路、新靶点，为最大程度减少由于个体差异而导致的再发不良心血管事件提供基础理论和研究依据。

急性冠脉综合征经皮冠状动脉介入治疗能显著提高患者的预后，但是仍有部分患者再次发生不良心血管事件，而不良心血管事件的复发可以由罪犯病变的复发所导致，也可能是因为快速进展的新发病变所引起。快速进展新发病变导致再发不良心血管事件的危害极大，但是对其机制性的探讨报道较少。已知转录因子TCF21和新型金属蛋白酶ADAMTS7基因可能与新发病变的快速进展相关。本项目从临床水平筛选出与快速进展新发病变相关基因TCF21与ADAMTS7的阳性多态性位点(ADAMTS7基因rs3825807)，进行基因克隆，体外表达，探讨多态性位点上的氨基酸改变对ADAMTS7基因编码蛋白结构和功能的影响，从而改变血管平滑肌细胞(VSMCs)的增殖和迁移，最终以临床验证为研究结点。本项目为急性冠脉综合征患者PCI术后再发不良心血管事件的防治提供一种新思路、新靶点，为最大程度减少由于个体差异而导致的再发不良心血管事件提供基础理论和研究依据。