NFKB通路与TGFB通路交互对话在升清降浊方治疗慢性肾功能衰竭中的作用及机制研究

Inflammation is one of the most important pathophysiological bases in chronic renal failure (CRF). Renal failure metabolic waste accumulates in the body, forming many pathological products. Preliminary study shows that Ascending Qing and Descending Zhuo Prescription has a significant effect on the treatment of CRF and can reduce the concentration of micro-inflammatory factors in peripheral blood and kidney tissue. Recent research shows that NF-κB signal pathway is one of the most important pathways of inflammation, that TGFβ/smad signal pathway is the critical path of renal fibrosis and NF-KB signal pathway has cross-talk with TGFβ/smad signal pathway and also NF-κB protein itself can cause muscle atrophy and malnutrition through inflammation. We think Ascending Qing and Descending Zhuo Prescription can affect inflammatory mediators of CRF, FKB and TGFB pathway. From the perspective of cell signal pathway, this project intends to research the effect of Ascending Qing and Descending Zhuo Prescription and its Chinese herb rhubarb and rhizoma cimicifugae on CRF and malnutrition, NF-κB and TGFβ/smad signal pathway to identity the influence of therapeutic targets to provide a theoretical basis for the Chinese medicine treatment of CRF.

炎症反应是慢性肾衰竭（CRF）重要的病理生理基础之一。肾衰状态下大量代谢废物在体内的蓄积，形成诸多病理产物。前期研究显示，升清降浊方治疗CRF 疗效确切，并可有效地降低外周血及肾组织微炎症因子的浓度。近期有研究显示：炎症反应中的重要通路之一为NF-KB信号通路，TGFβ/smad信号通路是肾脏纤维化的关键通路，NF-KB信号通路一方面与TGFβ/smad信号通路有交叉对话，NF-KB信号蛋白本身可通过炎症反应引起肌肉萎缩，营养不良。我们认为升清降浊方对慢性肾衰炎症介质及NF-KB通路及TGFB通路的影响存在关联？本课题从细胞信号通路角度探讨以升清降浊方及其主要单味药大黄、升麻对慢性肾衰竭及其营养不良的研究和NF-KB信号通路和TGFβ/smad信号的研究，以明确升清降浊方治疗CRF的作用靶点，为中医治疗CRF 提供理论依据。

炎症反应是慢性肾衰竭（CRF）重要的病理生理基础之一。肾衰状态下大量代谢废物在体内的蓄积，形成诸多病理产物。前期研究显示，升清降浊方治疗CRF 疗效确切，并可有效地降低外周血及肾组织微炎症因子的浓度。近期有研究显示：炎症反应中的重要通路之一为NF-KB信号通路，TGFβ/smad信号通路是肾脏纤维化的关键通路，NF-KB信号通路一方面与TGFβ/smad信号通路有交叉对话，NF-KB信号蛋白本身可通过炎症反应引起肌肉萎缩，营养不良。我们认为升清降浊方对慢性肾衰炎症介质及NF-KB通路及TGFB通路的影响存在关联？本课题从细胞信号通路角度探讨以升清降浊方及其主要单味药大黄、升麻对慢性肾衰竭及其营养不良的研究和NF-KB信号通路和TGFβ/smad信号的研究，以明确升清降浊方治疗CRF的作用靶点，为中医治疗CRF 提供理论依据。