智能型基因-化学药物共传输磁性纳米复合载体的构建及评价

The magnetic nanoparticles in the biomedical field have an important application prospect.But there is still a challenge about how to obtaine intelligent magnetic nanoparticles as drug carriers, which could simultaneously efficiently deliver chemical drugs and gene drugs, control the gene therapeutic drugs and chemical drugs release in tumor cells, super-sensitive recognize the cancer cells, diagnose cancer, report cancer cells death..This project aims to construct intelligent magnetic nano composite material as gene-chemical drug co-delivery carries. Fristly, the porous hollow Au-Fe3O4 nanoparticles will be designed and synthesized, and then Graphene oxide, Rare earth conversion nanoparticles and iRGD pepite targeting to liver cancer cells throught fuction will be modified on the surface of porous hollow Au-Fe3O4 nanoparticles. The chemical drugs are loaded in the hollow gold nanoparticles, and gene drugs are carried on the surface of Graphene oxide. This carrier could realize target identification of liver cancer cells, nondestructive in situ diagnosis of liver cancer, controlled release of drugs, combined therapy of liver cancer and real-time monitoring of the treatment process with visualization. Ultimately, they will radically change the way we diagnose, treat, and prevent cancer in oncology..The completion of the project will provide basic datas for the research and clinical experiment of nano drug controlled release system, and the important scientific basis for multimode diagnosis and treatment of cancer in the future.

磁性纳米粒子在生物医用领域具有重要的应用前景，但如何得到能高效运载基因药物和化学药物，对药物达到可控释放，超灵敏识别癌细胞，诊断癌症以及报告癌细胞的死亡等功能为一体的智能型磁性纳米药物载体，仍是当前面临的一大挑战。.本项目拟首先设计合成多孔空心金-四氧化三铁纳米粒子，然后分别将氧化石墨烯、靶向肝癌细胞的iRGD肽和稀土上转换纳米粒子通过功能化修饰在其表面，金纳米粒子内装化学药物，氧化石墨烯表面负载基因药物，构建出智能型的基因-化学药物共传输磁性纳米复合控释载体。这种载体可实现靶向识别肝癌细胞，无损原位诊断肝癌，可控释放药物，联合治疗肝癌并可视化实时监测治疗过程, 从而根本上改变肿瘤学诊断、治疗和预防肝癌的方式。.此项目的完成，将为纳米药物控释系统的研究和临床实验提供基础数据，并为今后多模式诊断和治疗癌症提供重要科学依据。

多功能纳米复合材料在生物医学领域如多种模式的生物影像、疾病的同时诊断治疗及药物控释等方面有广阔的应用前景。本研究在这一背景及申请的国家基金委项目的资助下在多功能纳米复合材料控制合成及其在载药、细胞成像等方面取得了一系列的进展。研究内容以申报的课题为指导，在金-四氧化三铁纳米粒子及相关的复合材料的合成、表面修饰组装、癌细胞的多模式诊断、检测方面以及药物载体和体内检测方面做了一系列工作。在课题进展过程中我们也对癌细胞检测的方法进行了创新。同时在项目实施过程中，我们也得到了荧光磁性纳米复合材料，并且发现得到的材料可以作为细胞中过量离子的检测和成像。本项目按照原计划取得了较好的成果，发表的SCI论文20篇，其中SCI一区12篇，SCI二区8篇。在该项目的支持下本人入选了教育部新世纪优秀人才，并得到了相应经费的支持。参与的国家自然基金重点项目和申报的国家基金面上项目获得了支持。项目按照原计划圆满完成，后继的一些延伸性的工作在继续探索中。