CaWRKY转录因子家族在辣椒灰霉病抗性应答中的调控机理研究

Gray mold caused by Botrytis cinerea is one of the most severe diseases in the production of pepper and other vegetable crops, WRKY transcription factors play important role in plant immunity system. However, the molecular mechanism of WRKY transcription factors in response to B. cinerea is still unclear. Our previous study demonstrated that pepper CaWRKYs may be involved in the response of desease resistance, and the transcript levels of CaWRKY genes were significantly different between B. cinerea resistant/susceptible lines, 6 candidate CaWRKYs potentially involved in B. cinerea response were identified. Thus, We proposed the hypothesis that CaWRKYs play critical roles in the transcriptional regulation of B. cinerea responding process in Pepper. Based on the characteristic analysis of 61 CaWRKYs, using technical means including qRT-PCR, VIGS and transgene, functional analysis of CaWRKYs were conducted. Furthermore, through yeast one/two-hybrid system, the interactions between CaWRKY and other components of B. cinerea response pathway were studied. In short, the implementation of this project would provide theoretical basis for the B. cinerea resisting regulation mechanism modulated by CaWRKY transcription factors in Pepper.

灰霉病是危害辣椒等蔬菜作物生产最严重的病害之一，WRKY转录因子在植物免疫进程中具有重要调控作用，然而目前WRKY转录因子在辣椒灰霉抗性应答中的调控机理还不清楚。申请人前期研究发现辣椒CaWRKY转录因子在抗病响应中发挥重要调控作用，并鉴定发现CaWRKY基因在辣椒灰霉抗/感材料中转录水平存在差异，进而筛选获得6个潜在参与辣椒灰霉应答调控的CaWRKY成员。基于此，提出CaWRKY参与辣椒灰霉病抗性转录调控的假设。本项目在分析辣椒全基因组61个CaWRKY蛋白/基因结构、系统进化等基本属性基础上，利用qRT-PCR、VIGS及转基因等技术研究CaWRKY在辣椒灰霉病抗性中的功能，并结合酵母单/双杂交技术分析CaWRKY与其它灰霉应答信号路径元件的相互作用，定位CaWRKY在调控辣椒灰霉胁迫响应中的关键信号节点。总之，本项目的实施将为解析CaWRKY参与调控辣椒灰霉病抗性机理奠定基础。

灰霉病是辣椒生产种植中最严重的病害之一，WRKY蛋白作为植物免疫进程重要调控作用因子，在辣椒灰霉抗性应答中的调控机理还不清楚。通过本项目的执行，在全基因组水平上对辣椒61个CaWRKY转录因子家族成员进行了系统研究，利用生物信息学手段对辣椒CaWRKY蛋白/基因结构、分类、染色体定位、系统进化等基本生物学信息进行了分析，利用qRT-PCR、VIGS及转基因等技术鉴定获得了对于辣椒灰霉病抗性具有显著调控功能的CaWRKY58，并对其灰霉病菌应答模式进行了分析与讨论。本项目的实施为解析辣椒中CaWRKY参与调控辣椒灰霉病抗性机理，挖掘更多抗病功能基因奠定了基础。