联合活体生物光学成像及超声微血管显像动态监测FGF及VEGF调控乳腺癌新生血管

Breast cancer is the most frequent cancer in Chinese women. Tumor angiogenesis associated with the diagnosis, treatment and prognosis in breast cancer patients. Fibroblast growth factors（FGF）and vascular endothelial growth factor (VEGF) associated with angiogenesis and increase the potential to the invasive, metastasis. FGF and VEGF signalling changes with different phases in tumor growth. And thus tumors may display a tremendous complexity and heterogeneity in the pattern of angiogenesis and tumor function. Bioluminescenece could quantitatively measure FGF and VEGF，a novel technique to visualize and characterize angiogenesis in vivo. We highlight novel observation on FGF and VEGF expression level with bioluminescenece in breast cancer angiogenesis in an animal model. By simultaneouly correlate bioluminescenece with contrast enhanced ultrasound microvascular imaging in different phase of tumor growth, we could investigate the angiogenesis process and its microvessel function as well as regulation of FGF and VEGF on angiogenesis. It could help to set up a study platform for evaluation to tumor angiogenesis and its in vivo.

乳腺癌已成为中国女性恶性肿瘤发病之首。肿瘤新生血管与乳腺癌诊断、治疗、预后各环节密切相关。成纤维细胞生长因子(fibroblast growth factors, FGF)、血管内皮生长因子( vascular endothelial growth factor, VEGF)与新生血管形成密切相关，同时参与肿瘤浸润、转移。在肿瘤不同生长阶段，上述因子的调节将使肿瘤新生血管的功能以及肿瘤代谢复杂化。 活体动物光学成像技术可定量测量FGF和VEGF，是体内生物学过程可视化、量化的崭新技术手段。本研究拟通过活体动物光学成像动态检测FGF和VEGF在肿瘤生长过程中的变化，同期超声微血管显像观察乳腺癌动物模型的肿瘤新生血管功能，通过二者实时对比探索乳腺癌肿瘤生长过程中FGF和VEGF对新生血管的调控机制，为乳腺癌在体肿瘤影像学功能评价提供研究平台。

乳腺癌已成为中国女性恶性肿瘤发病之首。肿瘤新生血管与乳腺癌诊断、治疗、预后各环节密切相关。成纤维细胞生长因子(fibroblast growth factors, FGF)、血管内皮生长因子( vascular endothelial growth factor, VEGF)与新生血管形成密切相关，同时参与肿瘤浸润、转移。在肿瘤不同生长阶段，上述因子的调节将使肿瘤新生血管的功能以及肿瘤代谢复杂化。活体动物光学成像技术可定量测量FGF和VEGF，是体内生物学过程可视化、量化的崭新技术手段。本实验通过基因工程技术，构建了能强制性表达bFGF，并携带报告荧光蛋白基因的乳腺癌细胞。在移植瘤生长期间，通过常规超声、超声造影以及荧光成像多种技术，纵向观测了肿瘤的生长、血供及bFGF表达情况。从细胞水平及动物水平，均验证了本模型用于研究乳腺癌血管生成与bFGF关系的可行性，为进一步的实验研究提供了良好的影像学平台支持。