黔产淫羊藿药材道地性及其主导因子研究

The geoherbalism of traditional Chinese medicine is the key of evaluation of traditional Chinese medicine quality and guarantee the clinical curative effect of traditional Chinese medicine. Herba Epimedii in Guizhou Province as the advantages of resources and genuine regional drug, its wide distribution, large output, wide application, but how to carry on the system evaluation of geoherbalism and the leading factors of the influence of geoherbalism is not clear. Accordingly,this project intend to Epimedium wushanense and E. acuminatum of representative in Guizhou ,from different level, such as botany phenotypic characterization, ecological factor, genetic differentiation, chemical composition spectrum and so on, to study geoherbalism quality of medicinal plants of Epimedium in Guizhou applied many kinds of methods, such as botany, molecular biology, chemical composition analysis, traditional Chinese medicine resources ecology, mathematical statistics and so on. By the correlation analysis of multiple factors, clarify the personality and common rules of its geoherbalism formation and the leading factors of the influence of its geoherbalism, preliminarily established diversified and comprehensive evaluation model of genuine regional Herba Epimedii to provide theory and technic supporting for the correlation problems of the production the development of medicinal materials.

中药的道地性是评价中药品质、保证中药临床疗效的关键。淫羊藿药材作为贵州省优势资源、道地药材，其分布广、产量大、应用广泛，但如何进行道地性系统评价及影响道地性的主导因子尚不清楚。立足于此空白，本项目拟以黔产具代表性的巫山淫羊藿、粗毛淫羊藿，从植物学表型特征、生态因子、遗传分化、化学成分谱等不同层面，应用植物学、分子生物学、化学成分分析、中药资源生态学、数理统计学等多种方法研究黔产淫羊藿属药用植物资源道地性品质。通过多因素的相关性分析，阐明其道地性形成的个性及共性规律和影响其道地性的主导因子，建立道地淫羊藿药材多元化、综合性的评价模式，为解决制约道地药材生产发展的相关问题提供理论和技术支撑。

淫羊藿作为贵州省重要的道地药材，具有补肾阳，强筋骨，祛风湿等功效，临床应用广泛，开展淫羊藿种质资源的系统评价研究，探寻淫羊藿药材道地性形成的主导因子对相关产业的持续健康发展非常重要。本项目通过对贵州主产的粗毛淫羊藿和巫山淫羊藿种质资源野外调查和样品收集保存，以分子生物学、植物形态学、中药资源生态学与中药分析学多学科结合，开展了淫羊藿种质资源多样性的系统评价，完成了二者原植物形态性状观测、药材活性成分含量测定、遗传多样性分析、环境因子查询与测定，以及淫羊藿种质资源的形态性状变异、活性成分含量变异与遗传因子和环境因子之间的相关性分析研究，总结了道地性形成的个性及共性规律和影响其道地性的主导因子，综合各项评价指标，筛选出粗毛淫羊藿优良种源6个，巫山淫羊藿优良种源2个，为解决制约道地药材生产发展的相关问题提供理论和技术支撑，为淫羊藿药材的良种选育、引种驯化、规范化种植及药材质量标准提升提供了研究基础。