食用植物油中多种危害因子混合污染快速同步检测研究

Aflatoxins and benzo-pyrene are important contamination factors which lead to threaten to edible vegetable oil consumption safety. There are few existing researches on simultaneous detection harmful factors in edible oils, and the existing detection methods for single factor are not effective enough. Before this research we have got monoclonal antibody with high quality to aflatoxins already, based on these achievements, we will prepare monoclonal antibodies against benzo-pyrene and others contamination factors. New kinds of immune signal particles are also need to be screened for binding monoclonal antibodies. After researches on the conditions for antibodies-signal particle binding and pre-processing of edible oil samples, a new platform for rapid simultaneously detection aflatoxins and benzo-pyrene in edible oils will be established base on the existing immunochromatographic methods. With this method, a rapid, visualized, high-performance, accurate and low-cost simultaneous detection on contamination factors in edible vegetable oil will come true. This study is an upgrade to the conventional colloidal gold immunochromatographic strip detecting method, and it is a breakthrough of the method for single-factor detection. This method will provide new ways for detection aflatoxins and other contamination factors in edible vegetable oils, and it is an enhancement to the development of immunology, analytical chemistry and material science. This research will offer a technological support for vegetable oil consumption safety.

黄曲霉毒素、苯并芘等是严重威胁食用植物油消费安全的主要危害因子，针对当前食用油危害因子同步快速分析研究很少，单因子检测效率低等不足，本研究拟在前期已获得黄曲霉毒素单克隆抗体的基础上进一步研制苯并芘等危害因子单克隆抗体，筛选包括纳米金在内的多种纳米信号材料，研究这些抗体与多种纳米信号材料的稳定偶联方法，探索食用植物油样品前处理手段，在现有免疫层析检测技术基础上，建立食用油中黄曲霉毒素、苯并芘等危害因子混合污染同步快速检测平台，实现食用植物油中黄曲霉毒素等危害因子直观、高效、准确、低成本的混合污染因子同步快速检测。本研究是对常规胶体金免疫层析试纸条检测平台的进一步提升，突破了单一信号标记的不足，该方法的建立将为食用植物油真菌毒素等危害因子的分析提供新的思路，为促进免疫学、分析化学、材料学等多学科交叉发展奠定坚实理论基础，为食品植物油安全消费提供关键技术支撑。

食用植物油是人类生活中不可或缺的重要食品，食用油消费安全直接关系到人类健康。目前，针对食用油等食品真菌毒素的污染问题，世界各国分别制定了严格的限量标准，并制定各种检测方法与标准。这些方法具有很好的特异性及灵敏度，但是组件复杂，造价较为昂贵，不利于现场检测应用普及。免疫纳米金技术具有操作简单、无需专业人员、成本低等，尤其适合现场检测等优点，已经渗透至食品安全检测的各个领域，本项目以食用植物油中黄曲霉毒素、赭曲霉毒素、玉米赤霉烯酮等严重威胁食用植物油消费安全的主要危害因子为突破口，针对食用油危害因子同步快速分析研究很少，单因子检测效率低等不足，在前期已获得黄曲霉毒素单克隆抗体的基础上进一步研制赭曲霉毒素、玉米赤霉烯酮等危害因子单克隆抗体，玉米赤霉烯酮抗体的灵敏度（IC50值）为0.02 ng/mL，赭曲霉毒素的灵敏度（IC50值）为0.058 ng/mL。建立纳米金稳定制备和标记技术，在现有免疫层析检测技术基础上，建立食用油中黄曲霉毒素、赭曲霉毒素、玉米赤霉烯酮等危害因子混合污染同步快速检测平台，可同步食用油中检测黄曲霉毒素B1、赭曲霉毒素A、玉米赤霉烯酮三种真菌毒素。三种真菌毒素之间无交叉反应，对黄曲霉毒素B1、赭曲霉毒素A、玉米赤霉烯酮的可视化检测限分别为0.25、0.5、1 ng/mL。实现食用植物油中黄曲霉毒素等危害因子直观、高效、准确、低成本的多元化同步快速检测。本研究对常规胶体金免疫层析试纸条检测平台的进一步提升，突破了单一信号标记的不足，方法的建立将为食用植物油真菌毒素等危害因子的分析提供新的思路，为促进免疫学、分析化学、材料学等多学科交叉发展奠定坚实理论基础，为食品植物油安全消费提供关键技术支撑。